Search

Luminescent bacteria–based biosensors are widely used for fast and sensitive monitoring of food safety, water quality, and other environmental pollutions. Recent advancements in biomedical engineering technology have led to improved portability, integration, and intelligence of these biotoxicity assays. Moreover, genetic engineering has played a significant role in the development of recombinant luminescent bacterial biosensors, enhancing both detection accuracy and sensitivity. This review provides an overview of recent advances in the development and applications of novel luminescent bacteria–based biosensors, and future perspectives and challenges in the cutting‐edge research, market translation, and practical applications of luminescent bacterial biosensing are discussed. [ABSTRACT FROM AUTHOR]

Bioluminescence refers to light produced by living organisms, including some species of bacteria of marine origin. The mechanism of bacterial luminescence is given by a biochemical reaction in which luciferin, oxygen, the enzyme luciferase and ATP participate, to result in the emission of light and the formation of water. The expression of the Lux genes that encode the enzymatic machinery is regulated for some species by quorum sensing, a phenomenon that allows light emission to only occur when there is a high cell density. Chemical and biological contaminants, as well as some physical factors in the environment can affect the luminescence emission. Given their sensitivity to environmental conditions and low toxicity, luminescent bacteria have been employed in a wide variety of fields, including gene expression analyses, drug discovery, studies of protein dynamics, mapping of signal transduction pathways and environmental monitoring. The objective of this review is to present the state of research on the phenomenon of bioluminescence, with a special focus on luminescent marine bacteria and their potential application in the field of biotechnology and environmental monitoring.. [ABSTRACT FROM AUTHOR]

Luminescent bacteria-based biosensors are widely used for fast and sensitive monitoring of food safety, water quality, and other environmental pollutions. Recent advancements in biomedical engineering technology have led to improved portability, integration, and intelligence of these biotoxicity assays. Moreover, genetic engineering has played a significant role in the development of recombinant luminescent bacterial biosensors, enhancing both detection accuracy and sensitivity. This review provides an overview of recent advances in the development and applications of novel luminescent bacteria-based biosensors, and future perspectives and challenges in the cutting-edge research, market translation, and practical applications of luminescent bacterial biosensing are discussed., (© 2024 John Wiley & Sons Ltd.)