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The plant Allium ampeloprasum, belonging to the Liliaceae family, is an important source of antioxidant compounds. In this study, the anti -radical and antioxidant activities, as well as the determination of phenolic and flavonoid compounds in the aqueous and alcoholic extracts of this plant, were investigated. In this laboratory study, the aqueous and alcoholic extracts of Allium ampeloprasum were prepared by adding 50 grams of the plant and mixing it in a 1:5 ratio with distilled water or ethanol. The total phenolic and flavonoid content was determined by spectrophotometry, and the antioxidant activity of the plant at different concentrations was measured using the Cuprac method, DPPH free radical scavenging, and ferric reducing antioxidant power (FRAP) assay. Data analysis was performed using SAS software and one -way analysis of variance. Based on the results, the total phenolic content in the aqueous extract was 86.9859 and 23.4 micrograms of gallic acid per milliliter in the alcoholic extract. The total flavonoid content was 42.81 in the aqueous extract and 345.54 micrograms of quercetin per milliliter in the alcoholic extract. Additionally, the total antioxidant capacity in the aqueous extract was 0.2362 and 0.3876 in the alcoholic extract, and the Cuprac method reported 0.0747 in the aqueous extract and 0.1992 in the alcoholic extract. The ferric reducing antioxidant power was 0.1041 in the aqueous extract and 0.0248 in the alcoholic extract, while the DPPH radical scavenging activity was 73.18 in the aqueous extract and 72.95 in the alcoholic extract. According to the study results, Allium ampeloprasum is a good source of antioxidant compounds, and its consumption can reduce oxidative damages in the body and improve health. Moreover, after extraction and purification, it can be used in pharmaceutical and food industries. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Faculty of Pharmacy of Ankara University / Ankara Üniversitesi Eczacilik Fakültesi Dergisi is the property of Ankara University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Carissa carandas (Apocynaceae), a renowned Ayurvedic plant, called as Karamarda in Sanskrit, and its fruits are reported to possess significant anti-inflammatory, antidiabetic, antiviral, antipyretic, and cardiotonic activities. The present investigation aimed for preliminary phytochemical, chromatographic studies and antioxidant activity of C. carandas fruits aqueous extract. Methods: Preliminary phytochemical studies, total flavonoid, and phenolic contents were established according to standard methods. Further, the aqueous extract was screened against free radicals, such as diphenyl picrylhydrazyl radical and nitric oxide radical. Further, its radical scavenging power was confirmed by phosphomolybdenum (total antioxidant activity) and reducing power assay. Results: C. carandas fruits aqueous extract showed significant antioxidant properties in nitric oxide (IC50-70 μg/ml), diphenyl picryl hydrazyl radical scavenging activity (IC50-800 μg/ml), and results are comparable with standard samples. Increasing concentration of fruit aqueous extract showed considerable reducing power and total antioxidant activity. The observed total phenols (50 mg GAE/g) and flavonoids (12 mg RE/g) present in aqueous extract is accountable for its antioxidant potential. High-performance thin-layer chromatography screening revealed that the aqueous extract is having different phytoconstituents with different Rf (Retention Factor) values and established the fingerprint profile for the identification of C. carandas fruits. Conclusion: The current phytochemical and chromatographic analysis can be considered reference standards for future studies on C. carandas fruits. Further study is required for the isolation of bioactive natural antioxidant from C. carandas fruits.

Background and Aims: Traditional indigenous system of medicine employed many Chenopodium species to treat a wide range of ailments. Therefore, the current study aims to evaluate the phenolic content, flavonoid content, and antioxidant capacity of the species Chenopodium album and Chenopodium pumilio. Methods: Phenolics and flavonoid content were quantified using spectrophotometric techniques. The antioxidant activity of Chenopodium extracts was evaluated by assaying ferric reducing antioxidant power, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, phosphomolybdenum reducing power, ferrous ion chelating activity, hydrogen peroxide radical scavenging activity, hydroxyl radical scavenging activity, deoxyribose degradation activity, and β-carotene bleaching activity. Results: In C. album and C. pumilio, total phenolic concentration was 17.5–23.8 and 13.3–25.3 mg tannic acid equivalent/g, respectively, and flavonoid content was 0.21–0.85 mg and 0.10–0.89 mg quercetin equivalent/g, respectively. Thus, C. pumilio had a higher content of phenolics and flavonoids than C. album. However, comparison of extracts in different solvents showed that ethanolic and aqueous extracts exhibited higher phenolics and flavonoid content than other solvent systems. The antioxidant capability of the species, determined by antioxidant assays, varied with the species evaluated and the solvents used for extraction. Remarkably, the correlation coefficient of different antioxidant assays with the phenolics and flavonoid content in both species showed discrepancy. Conclusion: Ethanolic and aqueous extracts of the Chenopodium species studied had higher phenolics, flavonoids, and free radical scavenging activity than methanolic and acetonic extracts. The ethanolic and aqueous extracts of the studied species provide a potential source of antioxidants for drug formulation. [ABSTRACT FROM AUTHOR]

Sophora exigua (SE) was sequentially extracted using hexane, ethyl acetate, and ethanol. The obtained extracts were tested for antioxidant activity. Among them, the fractionated ethyl acetate extract (SE-EA) showed the highest potential in free radical scavenging and ferric-reducing properties. The chemical analysis identified sophoraflavanone G as one of the active ingredients in SE-EA. According to SE-EA solubility, SE-EA liposomes were developed using a sonication-assisted thin film method. Cholesterol and phospholipids were used as the main compositions of the liposomes. The obtained liposomes were spherical with different nano-size ranges, size distribution, and zeta potential depending on SEEA and total lipid concentrations. SE-EA liposomes were slightly bigger than their empty liposomes. All liposomes exhibited a phospholipid crystalline structure. Cholesterol and SE-EA existed in the liposomes as an amorphous state. SE-EA liposomes with high total lipid content exhibited high entrapment efficiency and sustained release behavior. Whereas liposomes with low total lipid content showed low entrapment efficiency and fast-release behavior. All SE-EA liposomes showed stronger antioxidant activity than the non-entrapped SE-EA. In conclusion, SE-EA is a natural source of potent antioxidants. The developed SE-EA liposomes are a promising pharmaceutical formulation to efficiently deliver the active ingredients of SE-EA and are suitable for further study in vivo. [ABSTRACT FROM AUTHOR]

As known, dates are packed with antioxidants and bioactive compounds and provide various health benefits, as evidenced by their varying nitrite levels. Algerian dates, on the other hand, have not been thoroughly investigated for their bioactive compounds and overall antioxidant capacity. This research aims to tap into this potential by meticulously measuring total polyphenols, flavonoids, and condensed tannins in three popular Algerian varieties (Phoenix dactylifera L.) Ksiba, Hamraya, and Deglet Nour, and the determination of their antioxidant activity using (scavenging assays of 2,2- diphenyl-1-picryhydrazyl (DPPH) and 2,2′-Azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) (ABTS) radicals, reducing power, and total antioxidant capacity. The analysis showed that the three varieties of date fruits are rich in total phenolics with an amount ranging between 71±51 and 7975±389 mg of gallic acid equivalent (GAE).100 g-1 of dry weight (DW), the flavonoid amount ranged from 31±3 to 767±4 mg of quercetin equivalent (QE) 100 g-1 DW and condensed tannins between 6± 2 and 653 ±64 mg of catechin equivalent (CE) 100 g-1 dry DW. The antiradical activity was quite promising and ranged between 0.5 and 24 µg AAE mg-1 extract for DPPH and between 2 and 113 µg AAE mg-1 extract for ABTS, while the reducing power and total antioxidant capacity values ranged from 16 to 154 μg ascorbic acid (AAE) mg-1 and 39 to 68 μg AAE mg-1 extract respectively. The results of this study show that Algerian date fruit can be regarded as a potential natural source of antioxidants, with ethyl acetate serving as the best extractant solvent, resulting in higher polyphenol content and antioxidant activities. [ABSTRACT FROM AUTHOR]

Objective: To study the content and quality characteristics of three types of apple pectin (WSP, NSP, CSP) treated by different drying methods. Methods: Apple pectin was dried by hot air drying (60, 75, 90 ℃), vacuum freeze dryingn (−50 ℃) and natural drying. The effects of different drying methods on the browning degree of apple and the pectin content, protein content, total sugar content, antioxidant capacity and pectin structure of three types of apples were studied. Results: The browning degree of apple slices was the smallest under hot air drying at 60 ℃. The content of three different types of pectin was different. The content of WSP was the most, ranging from 58.06% to 77.09%, followed by NSP, ranging from 30.57% to 54.98%, and the content of CSP was the least, ranging from 14.64% to 27.06%. Among them, the content of three types of pectin after hot air drying at 60 ℃ was the most. The total sugar content of WSP, CSP and NSP was 34.56%~6.92%, 11.02%~4.04% and 15.46%~8.75%, respectively. The total sugar content of WSP dried at 75 ℃ was the highest. The total sugar content of NSP and CSP under hot air drying at 90 ℃ was also the highest. The protein content of three different types of pectin was different. The range of WSP, NSP and CSP was 2.92%~5.73%, 1.39%~4.27% and 1.50%~5.40%, respectively. Fourier transform infrared spectroscopy analysis showed that there was little difference in the structure of apple pectin under different drying conditions. The drying method had a significant effect on the antioxidant capacity of pectin. The antioxidant capacity of WSP and NSP ranged from 91.27% to 78.54% and 8.15% to 2.14%, respectively. The antioxidant capacity of CSP was very weak. Among them, the antioxidant capacity of WSP and NSP under 90 ℃ hot air drying was the strongest, which was 91.27% and 8.15%, respectively. The antioxidant capacity of WSP and NSP under freeze drying was the weakest, which was 78.54% and 2.14%, respectively. Conclusion: After apples were treated by different drying methods, the contents and properties of three different types of pectin in apples were different. The apple pectin dried at 60 ℃ was the best, and the apple pectin content, total sugar content, protein content and antioxidant capacity were 50.69%, 15.27%, 3.61% and 31.33%, respectively. This study would provide theoretical support for the extraction process of apple pectin and the antioxidant capacity of different types of pectin, and also provide some reference value for further study on the effect of different drying methods on pectin drying.

AbstractFor the first time, methanolic leaf extract of the hydrophyte Persicaria senegalensis L. was evaluated for its ability to silver nanoparticles (AgNPs) biosynthesis. The effect of stirring time and plant extract concentrations was studied. X-ray diffraction (XRD) and scanning electron microscope (SEM) were used for characterization of AgNPs. XRD confirmed the AgNPs formation with crystallite size in the range 23–71 nm. The crystallite size increased with increasing both stirring time and plant extract concentration. Antioxidants, including secondary metabolites such as flavonoids and phenols are substances that inhibit oxidative damage to the cell organisms by scavenging the free radicals and thus reducing the hazard of diseases. The scavenging and antioxidant activities of the plant leaf extract and AgNPs were evaluated and exhibited potent antioxidant activity. The total antioxidant capacity (TAC) of plant leaf extract and AgNPs was 52.41 and 10.13 µg AA equivalent/mg DW, respectively. Total phenolics (TPs) and total flavonoids (TFs), revealed high contents equal to 154.83 µg gallic acid equivalent and 100.05 µg quercetin equivalent/mg DW plant extract, respectively. [ABSTRACT FROM AUTHOR]

Copyright of Erzincan University Journal of Science & Technology is the property of Erzincan Binali Yildirim Universitesi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Different types of milk are used in the production of milk kefir, but little information is available on the release of potentially antioxidant exopolysaccharides (EPS). The aim of this study was to investigate whether the microbial dynamics and EPS release are dependent on the milk substrate. In our study, the inoculated microbial consortium was driven differently by each type of milk (cow, ewe, and goat). This was evident in the sugar consumption, organic acid production, free amino release, and EPS production. The amount and the composition of the secreted EPS varied depending on the milk type, with implications for the structure and functional properties of the EPS. The low EPS yield in ewe's milk was associated with a higher lactic acid production and thus with the use of carbon sources oriented towards energy production. Depending on the milk used as substrate, the EPS showed different monosaccharide and FT-IR profiles, microstructures, and surface morphologies. These differences affected the antiradical properties and reducing power of the EPS. In particular, EPS extracted from cow's milk had a higher antioxidant activity than other milk types, and the antioxidant activity was concentration dependent. [ABSTRACT FROM AUTHOR]

Changes in structural characteristics and antioxidant activity of tilapia hydrolysate glycated with glucose, fructose, or xylose at 90 °C for 12 h, and following in vitro gastrointestinal (GI) digestion were investigated. Fourier-transformed infrared (FTIR) band between 1,800 and 1,400 cm−1 confirmed the structural modifications of hydrolysate under glycations. Glycation drastically increased ATBS·+ and ONOO- scavenging activities (p

Different types of milk are used in the production of milk kefir, but little information is available on the release of potentially antioxidant exopolysaccharides (EPS). The aim of this study was to investigate whether the microbial dynamics and EPS release are dependent on the milk substrate. In our study, the inoculated microbial consortium was driven differently by each type of milk (cow, ewe, and goat). This was evident in the sugar consumption, organic acid production, free amino release, and EPS production. The amount and the composition of the secreted EPS varied depending on the milk type, with implications for the structure and functional properties of the EPS. The low EPS yield in ewe’s milk was associated with a higher lactic acid production and thus with the use of carbon sources oriented towards energy production. Depending on the milk used as substrate, the EPS showed different monosaccharide and FT-IR profiles, microstructures, and surface morphologies. These differences affected the antiradical properties and reducing power of the EPS. In particular, EPS extracted from cow’s milk had a higher antioxidant activity than other milk types, and the antioxidant activity was concentration dependent.

Plants are rich sources of therapeutic compounds that often lack the side effects commonly found in synthetic chemicals. Researchers have effectively synthesized pharmaceuticals from natural sources, taking inspiration from traditional medicine, in their pursuit of modern drugs. This study aims to evaluate the phenolic and flavonoid content of Solanum virginianum seeds using different solvent extracts, enzymatic assays including 2,2-diphenyl-1-picrylhydrazyl activity, reducing power, and superoxide activity. Our phytochemical screening identified active compounds, such as phenols, flavonoids, tannins, and alkaloids. The methanol extract notably possesses higher levels of total phenolic and flavonoid content in comparison to the other extracts. The results highlight the superior antioxidant activity of methanol-extracted leaves, demonstrated by their exceptional IC 50 values, which surpass the established standard. In this study, molecular docking techniques were used to assess the binding affinity and to predict the binding conformation of the compounds. Quercetin 3-O beta-d-galactopyranoside displayed a binding energy of -8.35 kcal/mol with several important amino acid residues, PHE222, TRP440, ILE184, LEU192, VAL221, LEU218, SER185, and ALA188. Kaempferol 3-O-beta-l-glucopyranoside exhibited a binding energy of -8.33 kcal/mol, interacting with specific amino acid residues including ALA 441, VAL318, VAL322, MET307, ILI409, GLY442, and PHE439. The results indicate that the methanol extract has a distinct composition of biologically active constituents compared to the other extracts. Overall, seeds exhibit promise as natural antioxidants and potential agents for combating cancer. This study highlights the significance of utilizing the therapeutic capabilities of natural compounds and enhancing our comprehension of their pharmacological characteristics., (© 2024 Wiley Periodicals LLC.)

The Folin-Ciocalteu assay is a tool widely employed to measure total phenolic content (TPC) in various applications across different fields, including food industry, agriculture, medicine, and environmental sciences. However, despite its popularity, there are limitations and an ongoing debate concerning its accuracy in TPC measurement. This review addresses these concerns within the context of phycology. A comprehensive database was compiled to quantitatively and qualitatively analyze the use of the Folin-Ciocalteu assay in this field. The quantitative approach led to the creation of an activity scale, indicating that brown algae, with their phlorotannins, and green microalgae stand out within the algal groups. Moreover, the qualitative approach indicates that the assay has proven to be a sensitive tool for studying algal ecology and physiology, and it has been widely employed to evaluate the biotechnological potential. However, a critical analysis reveals concerns related to interfering non-phenolic compounds and insufficient information on data reporting, including imprecise language and undefined terms, which hinders comparison with literature. Despite the growing interest in the Folin-Ciocalteu assay, most articles (98.7 %) do not take into account the effect of interfering compounds on TPC determination. Thus, the Folin-Ciocalteu assay is used as a measure of reducing power or antioxidant capacity in phycology rather than a measure of TPC. Nevertheless, with proper consideration of limitations, the Folin-Ciocalteu assay remains a valuable tool due to its simplicity, cost-effectiveness, and sensitivity. • Comprehensive database for Folin-Ciocalteu assay in phycology • Activity scale identifies prominent algal groups. • Sensitive tool: Studying ecology, physiology, and biotechnological potential • Concerns: Interfering non-phenolic compounds and data reporting • Valuable tool: Simplicity, cost-effectiveness, and sensitivity retained [ABSTRACT FROM AUTHOR]

Tortola bean leaves are an agricultural by-product from Tortola beans, which is widely consumed in Chile. Free, esterified, etherified, and insoluble-bound phenolics of Tortola bean leaves were evaluated for total phenolic content (TPC), antioxidant properties, and phenolic profile using UPLC–MS/MS for the first time, thus all results presented are novel considering previous literature. The soluble phenolics (free + esterified + etherified) presented higher TPC values (4667.03 μg gallic acid equivalent/g dry weight), reducing power (1474.26 mmol Trolox equivalent/100 g dry weight) and antioxidant activity towards peroxyl radicals (245.24 mmol Trolox equivalent/100 g dry weight). The free phenolics fraction showed the highest TPC and equal or higher antioxidant properties in chemical assays. Furthermore, it showed the highest phenolics content as evaluated by UPLC–MS/MS, regardless of the fraction (free, esterified, etherified, or insoluble-bound). Quercetin and rutin derivatives were the most abundant compounds (323.12–421.31 μg/g dry weight), mainly in the fraction containing free phenolics (50%). Free phenolic enriched in quercetin and their derivatives exhibited similar antioxidant protection to those of commercial quercetin in Caco-2 cells subjected to an oxidative challenge induced by exogenous hydrogen peroxide. Tortola bean leaves thus merit further consideration from academia and industry to develop nutraceuticals and/or functional foods. [ABSTRACT FROM AUTHOR]

Changes in structural characteristics and antioxidant activity of tilapia hydrolysate glycated with glucose, fructose, or xylose at 90 °C for 12 h, and following in vitro gastrointestinal (GI) digestion were investigated. Fourier-transformed infrared (FTIR) band between 1,800 and 1,400 cm -1 confirmed the structural modifications of hydrolysate under glycations. Glycation drastically increased ATBS · + and ONOO - scavenging activities ( p  < 0.05) as well as ferric-reducing antioxidant power (FRAP). Xylose was the most effective sugar for glycation, yielding the highest chemical antioxidant activities ( p  < 0.05). However, glycated hydrolysates exhibited lower cellular antioxidant activity (CAA) on HepG2 cell when compared to hydrolysates. The extensive glycation of hydrolysates resulted in lower GI digestibility as confirmed by the FTIR spectra of C[bond, double bond]O, C-N, N-H, C-C, C-O, and C-H stretching vibrations. Glycation of tilapia hydrolysates only improved chemical antioxidant activities, but alleviated CAA, especially upon simulated GI digestion., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)