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The utilization of food industry by-products represents a significant opportunity for developing functional foods. This study investigated the incorporation of Cucurbita maxima Plomo peel powder (PS) into wheat bread formulations to assess its potential as a valuable ingredient for bread-making. PS was incorporated into wheat flour at 1%, 10%, and 20% levels. The dough's rheological properties were analyzed using Mixolab. Bread samples were evaluated for physical characteristics (volume, texture, colour), antioxidant properties (DPPH, ABTS, FRAP), and reducing sugar content. Analyses were performed on day 0 and after 7 days of storage. PS incorporation significantly modified dough rheology, with increased development time and enhanced protein stability. Bread volume decreased progressively with PS addition (from 195.5 cm3 to 109.8 cm3 at 20% PS). However, antioxidant activity increased substantially, particularly in the crust, with ABTS values rising from 2.37 to 10.08 TE μM/g DM in water extracts. Total phenolic content and reducing sugars showed significant increases across all PS concentrations. Storage studies revealed stable antioxidant properties but progressive textural changes, with hardness increasing from 6.83 N to 108.8 N at 20% PS after 7 days. While PS incorporation affects bread's physical properties, the significant enhancement in antioxidant activity and phenolic content suggests its potential as a functional ingredient. The optimal incorporation level should balance technological properties with nutritional benefits. [ABSTRACT FROM AUTHOR]

Acrylamide formation in bread products poses health concerns, necessitating strategies to reduce its presence while maintaining nutritional value. This study investigated how different concentrations of pumpkin flour (Cucurbita maxima Plomo) and prolonged proofing times affect acrylamide content and bread characteristics. Composite bread samples were prepared with varying pumpkin flour shares (0–20%) to soft wheat flour using two proofing times (60 and 120 min). The study analyzed quality features, crust and crumb color, antioxidant activity, total polyphenolic content, reducing sugars, and acrylamide content of the resulting breads. Extended proofing (120 min) reduced acrylamide levels in the crust from 220 to 150 units in 20% pumpkin flour bread compared to 60 min proofing. Control bread showed the highest specific volume (2.40 ± 0.01 cm3/g) after 2 h of proofing, while 20% pumpkin flour addition decreased it to 1.69 ± 0.02 cm3/g. Initial hardness increased from 6.8 ± 1.5 N in the control to 14.3 ± 1.5 N in 20% pumpkin flour bread after 1-h of proofing. Water activity decreased from 0.966 ± 0.002 in the control to 0.945 ± 0.004 in 20% pumpkin flour samples with 2 h proofing. Optimal results were achieved with 5–10% pumpkin flour substitution combined with two-hour proofing, balancing improved nutritional properties and reduced acrylamide formation while maintaining acceptable bread quality parameters. [ABSTRACT FROM AUTHOR]