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Tunneling nanotubes (TNTs), submicrometer membranous channels that bridge and connect distant cells, play a pivotal role in intercellular communication. Organelle transfer within TNTs is crucial in regulating cell growth, signal transmission, and disease progression. However, precise control over individual organelle transport within TNTs remains elusive. In this study, we introduce an optical technique that harnesses TNTs as biophotonic conveyors for the directional transport of individual organelles between cells. By utilizing near-infrared light propagating along the TNTs, optical forces were exerted on the organelles, enabling their active transport in a predetermined direction and at a controlled velocity. As a potential application, TNT conveyors were employed to inhibit mitochondrial hijacking from immune cells to cancer cells, thereby activating immune cells and suppressing cancer cell growth. Furthermore, neural modulation was achieved by transporting mitochondria and neurotransmitter-containing vesicles between neurons via TNT conveyors and axonal conveyors, respectively. This study presents a robust and precise approach to immune activation and neural regulation through the manipulation of organelle transfer at the subcellular level.

Glaucoma causes dysfunction to tissues located in the anterior and posterior eye. In the anterior eye, the trabecular meshwork (TM) is the site of pathogenesis, where decreased TM cell numbers and alterations to the amount and composition of extracellular matrix hinder outflow of aqueous humor fluid from the anterior chamber. This causes intraocular pressure (IOP) elevation. Elevated IOP, a main risk factor for primary open-angle glaucoma, damages the axons of retinal ganglion cells in the posterior eye, which ultimately leads to blindness. Thus, clinical treatment paradigms for glaucoma are focused on reducing IOP. Normotensive IOPs are established by balancing the production of aqueous fluid from the ciliary body with drainage through the TM to Schlemm's canal. When IOP becomes elevated, TM cells coordinate a homeostatic response to lower IOP, which requires effective and efficient cellular communication. Tunneling nanotubes (TNTs) are transient specialized structures that allow cells to communicate with one another. Actin-rich tubes allow direct transmission of signals and cargoes between cells. This is important to overcome limitations of diffusion-based signaling in aqueous environments such as the anterior eye. Here, we describe a live-cell imaging method for monitoring TNTs in primary TM cells., (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)