1. Applications of optical resonance to biological imaging and label-free protein microarrays.
- Author
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Unlü MS, Ozkumur IE, Needham J, Bergstein DA, Goldberg BB, Yalcin A, Spuhler P, Irani R, and DeLisi C
- Subjects
- Animals, Biosensing Techniques, Electrochemistry instrumentation, Fluorescence, Fluorescent Dyes, Humans, Nucleic Acid Hybridization, Microscopy, Fluorescence instrumentation, Microscopy, Fluorescence methods, Optics and Photonics instrumentation, Optics and Photonics methods, Protein Array Analysis instrumentation, Protein Array Analysis methods, Proteins chemistry
- Abstract
We present biological imaging and sensing methods based on optical resonance and interference. In fluorescence microscopy, our nanoscale imaging capability sheds light onto conformational changes of DNA, DNA-protein complexes and polymer coatings on a solid surface. Interference measurements on a layered substrate yield a label-free sensing platform for protein binding in a high-throughput micro-array format.
- Published
- 2008
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