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Diabetic Retinopathy (DR) occurs due to Type II diabetes. At the early stage, if it is identified one can save their vision. Later stage, retinal detachment leads to 100% vision loss. An automatic computer based system is needed for diagnosis. There are diverse tools and automatic and semi-automatic systems are available. But the system is not identifying and measuring the narrow blood vessels accurately, because of the noise and imaging problems. Also, while tracking the retinal vessel, the narrow vessels are equally taken into consideration as wider vessels. Thus the proposed segmentation and classification techniques extract the blood vessels and measure the profile features of fundus images obtained from dissimilar modalities significantly.