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Stroma-free solutions of human haemoglobin modified with pyridoxal-5-phosphate, glutaraldehyde, borohydride and serum albumin were injected into the artery of an isolated rat heart perfused with Krebs-Henseleit solution under hypoxic conditions. About 70% of the oxygen transported by the modified haemoglobin was found to be utilized for a marked increase in the force of heart contraction. The results were in general correlation to the analysis of oxygenation curves of haemoglobin samples under study and confirmed the oxygen offloading ability.

Mouse cells were fractioned by adherence chromatography on glass beads. The fractionation of spleen cells yielded 5 different fractions, whose transplantation effect was tested by the method of Till and McCulloch. The results of these transplantation tests showed that the chosen form of adherence chromatography was not sufficiently successful, since none of the resultant fractions contained solely colony-forming units (CFU) and in none of them were CFU present in an exeptionally high concentration.