1. Dual-path platform (DPP) and enzyme-linked immunosorbent assay (ELISA): Change the sequence of the tests does not change the number of positive dogs for canine visceral leishmaniasis
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Larissa Pinheiro, Carla Lobo Gomes, Mauricio Oviedo Paciello, Kelvinson Fernandes Viana, Elaynne Costa Silva, Sara Santos Almeida, Luiz Carlos Bertucci Barbosa, Rodolfo Cordeiro Giunchetti, Sarah Tolentino Rocha Brandao, Wellida Patricia Aviz, Raimundo Wagner de Souza Aguiar, and Alex Sander Rodrigues Cangussu
- Subjects
0301 basic medicine ,chemistry.chemical_classification ,business.industry ,030231 tropical medicine ,Leishmaniasis ,Plant Science ,030108 mycology & parasitology ,medicine.disease ,Serum samples ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,Visceral leishmaniasis ,Enzyme ,chemistry ,Immunology ,Elisa test ,medicine ,Christian ministry ,business - Abstract
The Brazilian Ministry of Health determined in 2012 that the official protocol for diagnosis of Canine Visceral Leishmaniasis (CVL) would be the Dual-Path Platform (DPP) for screening, followed by enzyme-linked immunosorbent assay (ELISA) for confirmation. This study evaluated serum samples from 426 dogs from a region in northern Brazil. All samples were tested according to the Official Protocol and the sequence inverting (ELISA followed DPP). Regardless of the protocol adopted, prevalence (14.7%) has not changed. The approach using ELISA followed by DPP state that, the number of positive animals in screening was higher compared to the official protocol. Screen the ELISA test could be more appropriate. Key words: Canine visceral leishmaniasis, Dual-Path Platform (DPP), enzyme-linked immunosorbent assay (ELISA), tocantins.
- Published
- 2017
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