1. Candida albicans CTN gene family is induced during macrophage infection: homology, disruption and phenotypic analysis of CTN3 gene.
- Author
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Prigneau O, Porta A, and Maresca B
- Subjects
- Amino Acid Sequence, Animals, Blotting, Northern, Candida albicans cytology, Candida albicans genetics, Carnitine O-Acetyltransferase biosynthesis, Cell Line, Computational Biology, Gene Expression Regulation, Fungal, Glucose pharmacology, Mice, Molecular Sequence Data, Mutation, Peroxisomal Targeting Signal 2 Receptor, Peroxisome-Targeting Signal 1 Receptor, Phenotype, Receptors, Cytoplasmic and Nuclear genetics, Receptors, Cytoplasmic and Nuclear physiology, Sequence Homology, Amino Acid, Up-Regulation, Candida albicans enzymology, Carnitine O-Acetyltransferase genetics, Carnitine O-Acetyltransferase physiology, Genes, Fungal, Macrophages microbiology
- Abstract
We have isolated a Candida albicans gene, coding for a putative peroxisomal carnitine acetyl transferase (CTN) protein, which is up-regulated during macrophage infection. In the present study, we describe the disruption of CTN3 gene (previously called CAT3) to gain insight into its potential role during infection. The ability of disrupted Candida mutants to filament was affected by several solid media. Northern blot analysis revealed that CTN3 gene may be involved not only in conditions of cell starvation but also during the process of germination. In agreement with the putative peroxisomal localization of the corresponding protein, we observed a strong glucose repression of CTN3 gene and, on the contrary, high level of transcription by carbon sources that induce the formation of peroxisomal proteins. Furthermore, we showed the existence of two additional C. albicans CTN encoding sequences, which are also induced during macrophage infection.
- Published
- 2004
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