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Seashore invertebrates such as mussels are exposed to multiple bouts of pollution related to human activities. Plastic debris originating from land-based activities are a concerning issue as they may be fragmented in smaller pieces (microplastics, < 5 mm diameter) which have an excellent potential for uptake by a large variety of animals. Here, we set out to explore the whole transcriptome profiling of Mytilus galloprovincialis associated with temporal variability of microplastics concentrations. Mussels were submitted to (i) a single 18 days-exposure to a concentration of microplastics found during pollution events (4.6 E+5 polyethylene microbeads L -1 ), (ii) a recovery period to investigate the reversibility of microplastics effects and (iii) a repeated exposure to microplastics to evidence acclimation to microplastics pollution events. Overall, 18 days-exposure to microplastics was mostly associated with disruption of mussel global homeostasis resulting in the production of stress and immune-related proteins and as a consequence, a diminution of energy allocated to growth. During the recovery period, a contrasting response was observed with the activation of apoptotic processes and the up-regulation of immune-receptors and stress-related proteins (glutathione peroxidase, hsp70) in mussels previously exposed to microplastics. These divergent responses, suggest that the establishment of compensatory mechanism as an attempt to recover, is not sufficient to counteract physiological stress induced by the first exposure. Finally, the differences observed in gene expression between single and repeated exposures to microplastics suggest, under the experimental conditions tested, that mussels may be able to establish a stress-memory upon microplastics exposure., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

The population of the Chilean endemic marine gastropod Concholepas concholepas locally called "loco" has dramatically decreased in the past 50 years as a result of intense activity of local fisheries and high environmental variability observed along the Chilean coast, including episodes of hypoxia, changes in sea surface temperature, ocean acidification and diseases. In this study, we set out to explore the molecular basis of C. concholepas to cope with biotic stressors such as exposure to the pathogenic bacterium Vibrio anguillarum. Here, 454pyrosequencing was conducted and 61 transcripts related to the immune response in this muricid species were identified. Among these, the expression of six genes (CcNFκβ, CcIκβ, CcLITAF, CcTLR, CcCas8 and CcCath) involved in the regulation of inflammatory, apoptotic and immune processes upon stimuli, were evaluated during the first 33 h post challenge (hpc). The results showed that CcTLR, CcCas8 and CcCath have an initial response at 4 hpc, evidencing an up-regulation from 4 to 24 hpc. Notably, the response of CcNFKB occurred 2 h later with a statistically significant up-regulation at 6 hpc and 10 hpc. Furthermore, the challenge with V. anguillarum induced a statistically significant down-regulation of CcIKB between 2 and 10 hpc as well as a down-regulation of CcLITAF between 2 and 4 hpc followed in both cases by an up-regulation between 24 and 33 hpc. This work describes the first transcriptomic effort to characterize the immune response of C. concholepas and constitutes a valuable transcriptomic resource for future efforts to develop sustainable aquaculture and conservations tools for this endemic marine snail species., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Ocean acidification (OA) is one of the main consequences of increasing atmospheric carbon dioxide (CO 2 ), impacting key biological processes of marine organisms such as development, growth and immune response. However, there are scarce studies on the influence of OA on marine invertebrates' ability to cope with pathogens. This study evaluated the single and combined effects of OA and bacterial infection on the transcription expression of genes related to antioxidant system, antimicrobial peptides and pattern recognition receptors in the edible mussel Mytilus chilensis. Individuals of M. chilensis were exposed during 60 days at two concentrations of pCO 2 (550 and 1200 μatm) representing respectively current and future scenario of OA and were then injected with the pathogenic bacterium Vibrio anguillarum. Results evidenced an immunomodulation following the OA exposure with an up-regulation of C-type Lectin and Mytilin B and a down-regulation of Myticin A and PGRP. This immunomodulation pattern is partially counteracted after challenge with V. anguillarum with a down-regulation of the C-type lectin and Mytilin B and the up-regulation of Myticin A. In turn, these results evidence that pCO 2 -driven OA scenarios might triggers specific immune-related genes at early stages of infection, promoting the transcription of antimicrobial peptides and patterns recognition receptors. This study provides new evidence of how the immune response of bivalves is modulated by higher CO2 conditions in the ocean, as well one factor for the resilience of marine population upon global change scenarios., (Copyright © 2017 Elsevier Ltd. All rights reserved.)