1. Peripheral effects induced in BALB/c mice infected with DENV by the intracerebral route.
- Author
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Oliveira ER, Amorim JF, Paes MV, Azevedo AS, Gonçalves AJ, Costa SM, Mantuano-Barradas M, Póvoa TF, de Meis J, Basílio-de-Oliveira CA, Nogueira AC, and Alves AM
- Subjects
- Animals, Cerebrum pathology, Dengue pathology, Dengue Virus pathogenicity, Disease Models, Animal, Humans, Male, Mice, Mice, Inbred BALB C, Virulence, Cerebrum virology, Dengue virology, Dengue Virus physiology
- Abstract
The lack of an immunocompetent animal model for dengue mimicking the disease in humans is a limitation for advances in this field. Inoculation by intracerebral route of neuroadapted dengue strains in mice is normally lethal and provides a straightforward readout parameter for vaccine testing. However, systemic effects of infection and the immune response elicited in this model remain poorly described. In the present work, BALB/c mice infected by the intracerebral route with neuroadapted DENV2 exhibited several evidences of systemic involvement. DENV-inoculated mice presented virus infective particles in the brain followed by viremia, especially in late stages of infection. Infection induced cellular and humoral responses, with presence of activated T cells in spleen and blood, lymphocyte infiltration and tissue damages in brain and liver, and an increase in serum levels of some pro-inflammatory cytokines. Data highlighted an interplay between the central nervous system commitment and peripheral effects under this experimental condition., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2016
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