1. Siglec-1-positive plasmacytoid dendritic cells (pDCs) in human peripheral blood: A semi-mature and myeloid-like subset imbalanced during protective and autoimmune responses.
- Author
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Wilhelm TR, Taddeo A, Winter O, Schulz AR, Mälzer JN, Domingo C, Biesen R, Alexander T, Thiel A, Radbruch A, Hiepe F, and Gerl V
- Subjects
- Adult, Autoimmune Diseases immunology, Autoimmunity immunology, Case-Control Studies, Dendritic Cells drug effects, Dendritic Cells metabolism, Female, Flow Cytometry, Humans, Immunophenotyping, In Vitro Techniques, Interferon-alpha immunology, Microscopy, Confocal, Microscopy, Fluorescence, Middle Aged, Myeloid Cells immunology, Toll-Like Receptor 9 immunology, Young Adult, Dendritic Cells immunology, Influenza Vaccines pharmacology, Lupus Erythematosus, Systemic immunology, Sialic Acid Binding Ig-like Lectin 1 metabolism, Yellow Fever Vaccine pharmacology
- Abstract
Plasmacytoid dendritic cells (pDCs) play a central role in the pathogenesis of systemic lupus erythematosus (SLE) as IFN-α producers and promoters of T-cell activation or tolerance. Here, we demonstrated by flow-cytometry and confocal microscopy that Siglec-1, a molecule involved in the regulation of adaptive immunoresponses, is expressed in a subset of semi-mature, myeloid-like pDCs in human blood. These pDCs express lower BDCA-2 and CD123 and higher HLA-DR and CD11c than Siglec-1-negative pDCs and do not produce IFN-α via TLR7/TLR9 engagement. In vitro, Siglec-1 expression was induced in Siglec-1-negative pDCs by influenza virus. Proportions of Siglec-1-positive/Siglec-1-negative pDCs were higher in SLE than in healthy controls and correlated with disease activity. Healthy donors immunized with yellow fever vaccine YFV-17D displayed different kinetics of the two pDC subsets during protective immune response. PDCs can be subdivided into two subsets according to Siglec-1 expression. These subsets may play specific roles in (auto)immune responses., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2016
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