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Low spatial resolution is often cited as the most critical limitation of magneto- and electroencephalography (MEG and EEG), but a unifying framework for quantifying the spatial fidelity of M/EEG source estimates has yet to be established; previous studies have focused on linear estimation methods under ideal scenarios without noise. Here we present an approach that quantifies the spatial fidelity of M/EEG estimates from simulated patch activations over the entire neocortex superposed on measured resting-state data. This approach grants more generalizability in the evaluation process that allows for, e.g., comparing linear and non-linear estimates in the whole brain for different signal-to-noise ratios (SNR), number of active sources and activation waveforms. Using this framework, we evaluated the MNE, dSPM, sLORETA, eLORETA, and MxNE methods and found that the spatial fidelity varies significantly with SNR, following a largely sigmoidal curve whose shape varies depending on which aspect of spatial fidelity that is being quantified and the source estimation method. We believe that these methods and results will be useful when interpreting M/EEG source estimates as well as in methods development., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.) more...

The functional significance of resting state networks and their abnormal manifestations in psychiatric disorders are firmly established, as is the importance of the cortical rhythms in mediating these networks. Resting state networks are known to undergo substantial reorganization from childhood to adulthood, but whether distinct cortical rhythms, which are generated by separable neural mechanisms and are often manifested abnormally in psychiatric conditions, mediate maturation differentially, remains unknown. Using magnetoencephalography (MEG) to map frequency band specific maturation of resting state networks from age 7 to 29 in 162 participants (31 independent), we found significant changes with age in networks mediated by the beta (13-30 Hz) and gamma (31-80 Hz) bands. More specifically, gamma band mediated networks followed an expected asymptotic trajectory, but beta band mediated networks followed a linear trajectory. Network integration increased with age in gamma band mediated networks, while local segregation increased with age in beta band mediated networks. Spatially, the hubs that changed in importance with age in the beta band mediated networks had relatively little overlap with those that showed the greatest changes in the gamma band mediated networks. These findings are relevant for our understanding of the neural mechanisms of cortical maturation, in both typical and atypical development., (Copyright © 2018. Published by Elsevier Inc.) more...

Introduction: Radiotherapy is a potent treatment against breast cancer, which is the most commonly diagnosed cancer among women. However, the emergence of radioresistance due to increased DNA repair leads to radiotherapeutic failure. Applying polyphenols combined with radiation is a more promising method leading to better survival. Enterolactone, a phytoestrogenic polyphenol, has been reported to inhibit an important radioresistance signaling pathway, therefore we conjectured that enterolactone could enhance radiosensitivity in breast cancer. To assess this hypothesis, radiation response of enterolactone treated MDA-MB-231 and T47D cell lines and corresponding cellular mechanisms were investigated., Methods: Cytotoxicity of enterolactone was measured via MTT assay. Cells were treated with enterolactone before X-irradiation, and clonogenic assay was used to evaluate radiosensitivity. Cell cycle distribution and apoptosis were measured by flow cytometric analysis. In addition, DNA damages and corresponding repair, chromosomal damages, and aberrations were assessed by comet, micronucleus, and cytogenetic assays, respectively., Results: Enterolactone decreased the viability of cells in a concentration- and time dependent manner. Enterolactone significantly enhanced radiosensitivity of cells by abrogating G2/M arrest, impairing DNA repair, and increasing radiation-induced apoptosis. Furthermore, increased chromosomal damages and aberrations were detected in cells treated with enterolactone combined with X-rays than X-ray alone. These effects were more prominent in T47D than MDA-MB-231 cells., Discussion: To our knowledge, this is the first report that enterolactone is a novel radiosensitizer for breast cancer irrespective of estrogen receptor status. Authors propose enterolactone as a candidate for combined therapy to decrease the radiation dose delivered to patients and subsequent side effects., (Copyright © 2016 Elsevier Inc. All rights reserved.) more...