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The Zika virus (ZIKV) genome encodes a polyprotein that can be post-translationally processed into functional viral proteins. The viral protease is indispensable in the maturation of viral proteins. The Zika protease comprises of two components crucial for catalysis. The N-terminal region of NS3 contains the catalytic triad and approximately 40 amino acids of NS2B are essential for folding and protease activity. NS2B is a membrane protein with transmembrane domains that are critical for the localization of NS3 to the membrane. In this study, we expressed and purified full-length NS2B from ZIKV in E. coli. Purified NS2B was then reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. It was found that compared to wild type NS2B, NS2B C11S mutation in LMPG exhibited dispersed cross peaks in the 1 H 15 N-HSQC spectrum, thereby suggesting the feasibility for structural characterization using solution NMR spectroscopy., (Copyright © 2018 Elsevier Inc. All rights reserved.)

Background: Obtaining meaningful temperature for the human body requires identifying a body site that will provide reliable data across a large population. It is important to understand that skin temperature does not solely depend on body-core temperature and may be affected by other physiological and environmental factors. Currently, there is lack of empirical data in correlating facial surface temperature with body core temperature. Present IR systems in use at airports/immigration checkpoints have not been scientifically validated particularly in regards to the false-negative rate. As a result, they may create a false sense of security by underestimating the number of febrile (and possibly infected) individuals. This article evaluates the effectiveness of thermal scanner when it is being used for mass blind screening of potential fever subjects such as SARS or bird flu patients., Methods: Bio-statistics with regression analysis and ROC is applied to analyse the data collected (502) from the SARS hospital in Singapore and conclusive results are drawn from them. The results are vital in determining two very important pieces of information: the best and yet practical region on the face to take readings and optimal pre-set threshold temperature for the thermal imager., Results: (1) The thermal scanner can be used as a first line tool for the mass blind screening of hyperthermia, (2) the readings from the scanner suggest good correlation with the ear temperature readings, (3) an imager temperature threshold should be determined by the environmental factors, outdoor condition in particular, the physiological site offset and the performance characteristics of thermal imager to warrant the most accurate and reliable screening operation., Conclusions: The analysis suggested that the thermal imager used holds much promise for mass blind screening when the readings from a specific region have a good correlation with the ear temperature. From the regression analysis, the best reading is taken from the maximum temperature in the eye region, followed by the maximum temperature in the forehead region. With ROC analysis, a randomly selected individual from the fever group has a test value larger than that for a randomly selected individual from the normal group in 97.2% of the time. The test can distinguish between the normal and febrile groups and an optimum threshold temperature for the thermal imager can be found. The pre-set threshold cut-off temperature for the current thermal imager was found to be 36.3 degrees C with reference to the associated environmental condition. Any temperature readings that exceed this reading will trigger off the alarm and a thermometer will be used to verify the whether the person is having fever.