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The localization of papaverine (PAV) in micelles of zwitter-ionic N-hexadecyl-N, N-dimethyl-3-ammonio-1-propanesulfonate (HPS), cationic cetyltrimethylammonium chloride (CTAC), and anionic sodium dodecyl sulfate (SDS) in D2O was studied by 1H NMR and ESR in the presence and absence of 5-doxyl- or 12-doxyl-stearic acid. PAV, surfactants, and spin probes are characterized by restricted anisotropic motion in micelles. The rotational correlation time of doxyl fragment was in the range of 0.2 to 0.5 nanoseconds. Binding of PAV to micelles decreases the mobility of both probes, suggesting the localization of PAV inside the hydrophobic part of micelles near the micelle-water interface. According to the NOE data, the methoxy groups of PAV are located in the vicinity of the nitrogen atom in CTAC and HPS micelles, the methoxy groups of the PAV heterocycle being immersed slightly deeper inside the micelle. The T1 relaxation enhancements by two different spin probes show that the H5 and methoxy substituents of the PAV heterocycle are in close proximity to the alpha-CH2 of acyl chains in all types of micelles, whereas H3 and H12 are the most distant from the alpha-CH2. No significant differences were found for the protonated and neutral PAV in SDS micelles at pD 4.9 and 11.2. These data show that the geometry of the PAV-micelle complex is practically independent of the PAV charge and surfactant head-group.