1. First study in cryopreserved Crassostrea angulata sperm
- Author
-
Elsa Cabrita, Sandra Joaquim, Domitília Matias, Marc Suquet, Marta F. Riesco, Francisca Félix, Centro de Ciências do Mar [Faro] (CCMAR), Universidade do Algarve (UAlg), Instituto Português de Investigação do Mar e da Atmosfera (IPMA), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)
- Subjects
Male ,0301 basic medicine ,Ethylene Glycol ,Oyster ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Portuguese oyster ,Aquaculture ,Broodstock ,Cryopreservation ,Cryoprotective Agents ,Endocrinology ,Freezing ,Pacific oyster ,education.field_of_study ,Crassostrea angulata ,biology ,Sperm Agglutination ,food and beverages ,Gigas ,Motility ,04 agricultural and veterinary sciences ,Spermatozoa ,Quality ,Horticulture ,Viability ,[SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies ,Sperm Motility ,Virginica ,animal structures ,Cryoprotectant ,Population ,03 medical and health sciences ,Semen ,biology.animal ,Botany ,Animals ,Dimethyl Sulfoxide ,14. Life underwater ,Crassostrea ,education ,Methanol ,ACL ,biology.organism_classification ,Sperm ,030104 developmental biology ,Fertilization ,040102 fisheries ,Oocytes ,0401 agriculture, forestry, and fisheries ,Animal Science and Zoology ,Semen Preservation - Abstract
Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For this reason, in this work a successful sperm cryopreservation protocol for this important species has been developed for the first time. Different internal cryoprotectants (DMSO, ethylene glycol, polyethylene glycol and methanol) at several concentrations (5, 10, 20%), containers (straws vs cryovials) and freezing rates (slow and fast rates) were tested. Cryoprotectant toxicity tests corroborated that this assay did not take into account the following steps of cryopreservation protocol as sperm agglutination. A fast freezing rate of cells diluted in 10% DMSO and the use of straws as containers were the best cryopreservation conditions for Portuguese oyster sperm. Finally, fertilization assays confirmed the efficiency of the cryopreservation protocol in oyster sperm. These results demonstrated that different susceptibilities have been detected concerning sperm cryopreservation depending on oyster species or genetic material composition. (C) 2016 Elsevier Inc. All rights reserved. Promar Program [CRIOBIV-31-03-05-FEP-59] CRIOBIV project info:eu-repo/semantics/publishedVersion
- Published
- 2017
- Full Text
- View/download PDF