1. Design, synthesis and biological evaluation of dual-function inhibitors targeting NMDAR and HDAC for Alzheimer's disease.
- Author
-
He, Feng, Ran, Yingying, Li, Xiaoyang, Wang, Defeng, Zhang, Qiuqiong, Lv, Jiahui, Yu, Chenggong, Qu, Ying, Zhang, Xiangna, Xu, Ana, Wei, Chao, Chou, C. James, and Wu, Jingde
- Subjects
- *
ALZHEIMER'S disease , *BIOSYNTHESIS , *METHYL aspartate receptors , *AMYLOID beta-protein precursor , *AMYLOID beta-protein , *HISTONE deacetylase inhibitors , *MOIETIES (Chemistry) , *HYDROGEN peroxide - Abstract
• Compound 9d with memantine as a cap structure exhibits balanced anti-NMDAR and HDAC activity. • The structural activity relationship is explained in detail. • HDAC inhibitors of isohydroxamic showed a strong protective effect against hydrogen peroxide damage. Histone deacetylases (HDACs) have been indicated important roles in neurodegenerative disorders including Alzheimer's disease (AD). Herein, a series of novel compounds that contain a memantine moiety were designed to target HDACs and N -methyl- d -aspartate receptor (NMDAR) which are related to the treatment of AD. Biological characterization established that compound 9d exhibited a balanced inhibitory activity on NMDAR and HDACs. This compound is relatively selective to HDAC6 with IC 50 of 0.18 μM and also maintains comparable activity on NMDAR (K i = 0.59 μM) as memantine. Functionally, treatment with 9d increased the level of AcTubulin in MV4-11 cells and rescued PC-12 cells from H 2 O 2 -induced cytotoxicity with EC 50 of 0.94 μM. Studies in mice also demonstrated that compound 9d efficiently penetrates the blood brain barrier to reach the brain tissue. Collectively, the results strongly encourage further development of 9d as a potential therapeutic agent for AD. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF