1. Adapting collagen/CNT matrix in directing hESC differentiation
- Author
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Sridharan, Indumathi, Kim, Taeyoung, and Wang, Rong
- Subjects
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COLLAGEN , *CARBON nanotubes , *EMBRYONIC stem cells , *CELL differentiation , *COMPOSITE materials , *ATOMIC force microscopy - Abstract
Abstract: The lineage selection in human embryonic stem cell (hESC) differentiation relies on both the growth factors and small molecules in the media and the physical characteristics of the micro-environment. In this work, we utilized various materials, including the collagen–carbon nanotube (collagen/CNT) composite material, as cell culture matrices to examine the impact of matrix properties on hESC differentiation. Our AFM analysis indicated that the collagen/CNT formed rigid fibril bundles, which polarized the growth and differentiation of hESCs, resulting in more than 90% of the cells to the ectodermal lineage in Day 3 in the media commonly used for spontaneous differentiation. We also observed the differentiated cells followed the coarse alignment of the collagen/CNT matrix. The research not only revealed the responsiveness of hESCs to matrix properties, but also provided a simple yet efficient way to direct the hESC differentiation, and imposed the potential of forming neural-cell based bio-devices for further applications. [Copyright &y& Elsevier]
- Published
- 2009
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