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Cocaine hydrochloride salts are one of the most commonly used drugs of our days, yet there is very little hard evidence regarding when people started consuming such an extensively popular drug in Europe. In this paper, we report the exceptional finding of Erythroxylum spp. in human remains dated to the 1600's in Milan, Italy. Toxicological analyses were performed on preserved human brains revealing the first evidence of Erythroxylum spp. use in Europe before the 19th century, backdating our understanding of the presence of the plant by almost two centuries. Specifically, the alkaloid of cocaine was detected in two separate biological samples and can be associated to Erythroxylum spp. consumption. Given that the plant was not listed inside the detailed hospital pharmacopeia, it may not have been given as a medicinal remedy but may have been used for other purposes. This study demonstrates the importance and the potential of the application of toxicological analyses to archaeological contexts and allows to backdate the arrival of the Erythroxylum spp. in Europe by almost two hundred years. • The paper presents the first evidence of Erythroxylum spp. in Europe in the 1600s. • 17th century brain tissue with active components of coca plant was found. • Archaeotoxicology backdates Erythroxylum spp. use by almost two centuries in Europe. [ABSTRACT FROM AUTHOR]

The identification of unarticulated human remains with anthropic marks in archaeological contexts normally involves solving two issues: a general one associated with the analysis and description of the anthropic manipulation marks, and another with regard to the interpretation of their purpose. In this paper we present new evidence of anthropophagic behaviour amongst hunter-gatherer groups of the Mediterranean Mesolithic. A total of 30 human remains with anthropic manipulation marks have been found in the Mesolithic layers of Coves de Santa Maira (Castell de Castells, Alicante, Spain), dating from ca. 10.2–9 cal ky BP. We describe the different marks identified on both human and faunal remains at the site (lithic, tooth, percussion and fire marks on bone cortex). As well as describing these marks, and considering that both human and faunal remains at the site present similar depositional and taphonomic features, this paper also contextualizes them within the archaeological context and subsistence patterns described for Mesolithic groups in the region. We cannot entirely rule out the possibility that these practices may be the result of periodic food stress suffered by the human populations. These anthropophagic events at the site coincide with a cultural change at the regional Epipalaeolithic-Mesolithic transition. [ABSTRACT FROM AUTHOR]

This paper approaches, from a computational perspective, the problem of predicting the stature of human skeletal remains from bone measurements. There are traditional methods for constructing models that give good results for stature estimation. In this paper, we aim to investigate the usefulness of using machine learning-based models to approximate stature. Assuming that the stature of an individual is indirectly related to bone measurement values, we can derive methods that learn from archaeological data and construct models that give good estimates of the stature. Two novel machine learning-based regression models for stature estimation are proposed in this paper. Experiments using artificial neural networks and genetic algorithms were performed on samples from the Terry Collection Postcranial Osteometric Database, and the obtained results are discussed and compared with the results from other similar studies. The experimental evaluations indicate that the machine learning-based regression models are efficient for the stature estimation of archaeological remains and highlight the potential of our proposal. [ABSTRACT FROM AUTHOR]

In this paper, we reported the results obtained from toxicological investigations on bone samples collected from human remains of the 17th century in Milano (Italy). The aim of this study was to search for analytical signs of the administration of medical or recreative plants in the population of Milano during the 17th century. Nine femoral bone samples were extracted via Solid-Phase Extraction and analyzed via Thermo Scientific™ TSQ Fortis™ II Triple-Quadrupole Mass Spectrometer. As a result, archeotoxicological analyses revealed the presence of two cannabinoids (Delta-9-tetrahydrocannabinol and cannabidiol) of the cannabis plant present in two out of nine bone samples (that correspond to 22% of the biological samples analyzed). The presence of these two alkaloids evidences the use of the cannabis plant in the Italian population during the 17th century. The documentation related to the pharmacopeia used to treat patients in the Ca' Granda is still present in the archives of the hospital. However, this plant was not listed inside the pharmacopeia, suggesting that the plant was not administered as medical treatment at the time in the hospital. Thus, we hypothesize that the subjects under investigation used cannabis as a recreational substance, although other sources of exposure, such as self-medication, administration as a medical plant by other doctors outside of the Ca' Granda, occupational and involuntary exposure, cannot be excluded. In conclusion, this study reports the first physical evidence of cannabis use in Modern Age in Italy but also in Europe. • Toxicological analyses on archaeological human remains. • Findings of Delta-9-THC and CBD in bone samples of 17th century. • Likely recreational use of cannabis plant in 1600's. • Archaeotoxicology a new scientific discipline in the field of research. [ABSTRACT FROM AUTHOR]

• Case study of war in the ancient Maya kingdom of Piedras Negras. • Settlement location, fortifications, weapons, human remains, and epigraphy. • Chronological changes in violence across the first millennium A.D. • War and processes of polity formation and collapse. • Evidence for total war and attacks on community. Through a case study of the Classic period (A.D. 350–900) kingdom of Piedras Negras, this paper addresses a number of debates in the archaeology of war among the ancient Maya. These findings have broader comparative use in ongoing attempts to understand war in the precolonial Americas, including the frequency of war, its role in processes of polity formation and collapse, the involvement of non-elites in combat, and the cause and effect of captive-taking. This paper provides the first synthesis of a number of datasets pertaining to war and violence in the region of Piedras Negras while presenting new settlement data gleaned from recent lidar survey of the area. Focus is especially on tracing the material, iconographic, and epigraphic evidence for war in diachronic perspective. Material evidence includes the spatial distribution of settlement, presence of fortifications, weaponry, and human skeletal remains demonstrating evidence of traumatic injury. Additional data are drawn from epigraphy and iconography. As with all archaeological contexts, there are crucial gaps in the record. Nevertheless, by combining these datasets it is possible to reconstruct a history of warfare within this precolonial indigenous polity of the first millennium. [ABSTRACT FROM AUTHOR]

Highlights • Intensive surface collection can produce size estimates for cremation cemeteries. • A size estimate and error range for the living is produced using several parameters. • Population catchments are modelled based on cemetery size and regional survey data. Abstract Disposal of the dead is a multi-step process usually involving several people and multiple locations over time. Understanding the size and internal composition of a cemetery generated by this process is important for estimating the social and chronological patterning of the people buried there. When we know the number of dead in a cemetery, the length of time the cemetery was in use, and the life expectancy of the population, we can also help establish the size and composition of the living community who used it. This paper provides both local and regional spatial perspectives for a mortuary population by combining surface collection and excavation to describe activity areas and population size at a Bronze Age cemetery in Eastern Hungary. While prehistoric features and activity areas are difficult to identify using surface collection alone, we find that the combination of surface collection and excavation data in a GIS permits us to estimate the size of the cemetery, propose plausible models of activity areas within it, and generate new hypotheses about the regional community that produced it. [ABSTRACT FROM AUTHOR]

Abstract: In recent papers, Coltrain et al. (Sealing, whaling and caribou: the skeletal isotope chemistry of eastern Arctic foragers, Journal of Archaeological Science 31, 39–57) and Coltrain (Sealing, whaling and caribou revisited: additional insights from the skeletal isotope chemistry of eastern Arctic foragers, Journal of Archaeological Science 36, 764–775) propose that four previously excavated human skeletons from Native Point, Southampton Island, Nunavut, Canada, are Dorset Paleoeskimo and European. The suggested reclassification of one burial as Dorset is based upon a single radiocarbon assay and interpretation of isotopic values. The authors’ conclusion that three other individuals, found together inside an aboriginal dwelling, were European rests on an isotopic signature indicative of a terrestrial diet, as well as radiocarbon date ranges. This paper assesses the information used by and to reach these conclusions and, in contrast to those authors, determines that the data is equivocal at best and does not support revision of the individuals’ biocultural affinity. The paper stresses the necessarily critical role that must be given to archaeological context in any interpretation, or reinterpretation, of human remains. [Copyright &y& Elsevier]

Abstract: We explore the standards of research and reporting needed to justify the destructive analysis of archaeological human bone for biomolecular studies of ancient tuberculosis (TB). Acceptable standards in osteological interpretation have been met in some biomolecular papers, but there are also cases where insufficient care has been taken in distinguishing between pathognomonic lesions and those that are ‘consistent with’ a diagnosis of TB. Some biomolecular studies have failed to recognize that archaeological bones might be contaminated with environmental mycobacteria whose DNA could give rise to false positives in polymerase chain reactions directed at members of the Mycobacterium tuberculosis complex. The difficulties of applying spoligotyping to ancient DNA have also been underestimated and conclusions drawn from such analyses are often weakly supported. Assumptions that mycobacterial DNA preserves better than human DNA, and that contamination with modern DNA is less of a problem, has led in some cases to a laxity in research standards with insufficient attention paid to the need to authenticate ancient DNA results. We illustrate our concerns by reference to a recent paper reporting biomolecular detection of ancient TB DNA in skeletons from the eastern Mediterranean Neolithic settlement of Atlit-Yam. We are unconvinced that the skeletal evidence presented in this paper gives sufficient indication of TB to warrant destructive analysis, and we are concerned that during the biomolecular part of the project inadequate attention was paid to the possibility that results might be due to laboratory cross-contamination or to amplification of environmental mycobacterial DNA present in the bones. [Copyright &y& Elsevier]

Abstract: In 1854 Chief Mugombane and members of his Kekana Ndebele chiefdom took refuge in what is now known as Historic Cave, in the Makapan Valley, South Africa. The chiefdom retreated into the cave following an attack on a party of Trekboers. The subsequent siege of the cave, which lasted just under a month, ended in the death and surrender of its occupants and their dispersal among the Trekboers and their African auxiliaries. However, the number of people and the composition of the group that hid in the cave, as well as factors that led to the demise of the Kekana became blurred in the various renderings of the historical event. During the 1980s researchers began to cast doubt on the original Trekboer documents and the magnitude of the event when it became apparent that the Kekana oral histories remained silent on the matter. Between 2001 and 2007 the material remains of the siege were excavated to provide new data from which to judge the scale and impact of the siege event. Human remains, the primary focus of this paper, afford this perspective. This paper provides the result of the analysis of the human remains excavated from the cave, as well as an account of those remains that have been recorded or are housed in collections. The study of two naturally mummified individuals removed from the caves and stored in collections is also presented. [Copyright &y& Elsevier]

Abstract: The Middle to Upper Paleolithic transition is a key period of change in the prehistory of the Old World and one of the most studied issues in paleoanthropology, as the nature of the transition(s) is still, after at least a century of archaeological research, largely unknown. Many of the issues at stake in the transition relate to the problem of building a reliable chronology for this period, which is at the limits of the radiocarbon method. The papers in this volume show that much progress has been made in our chronological knowledge of significant aspects of the transition, such as the age of the most recent Neandertal fossils and the earliest modern human remains in Europe, and the inferred overlap between the Châtelperronian and the Aurignacian. At the same time, the volume also shows where the chronological database for the period 40 to 30ka 14C BP is flawed and that significant contextual and methodological problems have been underestimated in a number of studies of the biological and cultural changes during this crucial period. Chronology is employed by paleoanthropologists to relate the record of the Middle to Upper Paleolithic transition to major biological and cultural developments. This paper takes a broader paleoanthropological perspective and attempts to evaluate and, to some degree, synthesize the main results of these proceedings, while also presenting a brief discussion of the Middle and Upper Paleolithic archaeological and fossil records, and possible explanations for the differences between the two, focusing on the role of differences in the ecology of Neandertals and early European modern humans. [Copyright &y& Elsevier]

Predictive policing, that is, the data-driven deployment of police operations on the ground, has become increasingly important in recent years. While predictive policing instruments serve to formalise the ways in which police think and operate, the human agent remains central to their exploitation and translation into strategic, operational, and tactical decision-making. The introduction of predictive policing instruments and methods therefore represents a particularly insightful terrain on which to analyse negotiations of formality and informality. How this plays out in the Swiss context will be addressed in this paper. Based on a review of documents and policies on predictive policing developments and exploratory interviews conducted with police officers and developers, we discuss how institutions and actors engage with predictive policing and what this tells us about the formalisation, respectively informalisation of police work. Our findings point to the challenges related to the federalist organisation of police in Switzerland and the growing importance of cantonal threat management (Bedrohungsmangement) platforms. We also note a general lack of awareness regarding the potentially harmful outcomes of predictive policing instruments, which may be related to a specifically Helvetic narrative that downplays the impact these instruments have on decision-making. [ABSTRACT FROM AUTHOR]

Lead (Pb) concentration and isotope analyses in human remains are powerful analytical tools used to examine differences in Pb exposure over time or between populations and to examine the movement of peoples and Pb-containing cultural materials. While there was a large increase in the use of dental tissues for measuring Pb in the last 30 years, there has yet to be a critical evaluation of how these analyses are conducted or the data are used to answer archaeological questions. This article reviews 55 papers published between 1979 and 2021 on Pb concentrations and isotope analyses of teeth from archaeological populations to examine how they were used, areas where they were misused or insufficient information given, current limitations of the approaches, and future studies needed. This was applied across three broad topics: sample choice, concentration interpretation, and isotopic ratio interpretation. While major limitations exist, largely related to missing information in methodological approaches, there are a few overarching themes of use that need to be considered. First is considering the biological/cultural age of the teeth and therefore of the individuals represented, and the variability introduced when comparing disparate tooth types. Second, is the need to consider archaeological, ethnographic, and historical documentation when evaluating natural versus anthropogenic exposures. Finally, we recommend that greater consideration of the contributions from both environmental and cultural sources, including those that could be imported into different cultural regions. The consideration of all these factors is integral to future studies involving Pb concentration and isotope analyses in bioarchaeology. • Sample choice, preparation, and analytical technique details are often missing • Roles of natural versus anthropogenic sources should be assessed using archaeological, ethnographic, and historical contexts • Both imported and locally produced cultural material sources must be considered when interpreting Pb exposure [ABSTRACT FROM AUTHOR]

• Maize consumption record from the northeast Patagonia is presented. • Archaeobotanical data is discussed with the oxygen isotopes (δ18O) on human remains. • Maize-consumers were local individuals who also consumed local wild plants. • Maize would have entered through exchange, probably from southern Andes. This paper discusses the maize consumption record among hunter-gatherers outside assumed production areas in northeastern Patagonia. We evaluated if this anomalous record is the result of occasional events of local production/consumption; the transport of the microremains in the teeth of individuals after consuming maize in non-local production areas; or the local consumption of maize after its transport/exchange from production areas. Archaeobotanical results showed that analyzed individuals, including maize-consumers, mainly consumed local wild plants. Maize was not cultivated locally, and its consumption was unusual but not extraordinary in northeastern Patagonia. Oxygen isotope values of analyzed individuals are strongly compatible with local water sources, which imply that the mobility range of them must have not exceeded extra-Andean North Patagonia. For this reason, the most plausible explanation for the presence of maize in the local archaeological record is that this plant to have entered northeastern Patagonia through exchange, probably from southern Andes (central Chile or central-west Argentina). [ABSTRACT FROM AUTHOR]

Excavations conducted by a Bulgarian-French team at Kozarnika Cave (Balkans, Bulgaria) during several seasons yielded a long Paleolithic archaeological sequence and led to the discovery of important faunal, lithic, and human samples. This paper aims to describe the unpublished radius shaft of an infant who died approximately before the sixth month postnatal that was recovered from layer 10b, which contained East Balkan Levallois Mousterian with bifacial leaf points. The layer was dated between 130 and 200 ka (large mammals biochronology) and between 128 ± 13 ka and 183 ± 14 ka (OSL), i.e. OIS6. Here we show that, given the scarcity of Middle Pleistocene infant remains in general, and Middle Paleolithic human remains from this part of Eastern Europe in particular, the study of the Kozarnika specimen is of special interest. We discuss its place in the Middle Pleistocene European hominine record and substantiate the hypothesis of early Neanderthal presence in the eastern Balkans. [ABSTRACT FROM AUTHOR]

Skeletal remains of two equid species, Equus caballus (horse) and Equus asinus (donkey), have been found in archaeological contexts throughout Iberia since the Palaeolithic and Chalcolithic periods, respectively. These two species play different economic and cultural roles, and therefore it is important to be able to distinguish between the two species to better understand their relative importance in the past human societies. The most reliable morphological features for distinguishing between the two domesticated equids are based on cranial measurements and tooth enamel folds, leading to only a small percentage of archaeological remains that can be identified to species. Ancient DNA (aDNA) analysis can be used to reliably distinguish the two equids, but it can be cost prohibitive to apply to large assemblages, and aDNA preservation of non-cranial elements is often low. Collagen peptide mass fingerprinting by matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry, also known as zooarchaeology by mass spectrometry (ZooMS), is a minimally destructive and cost-effective alternative to aDNA analysis for taxonomic determination. However, current ZooMS markers lack resolution below the genus level Equus. In this paper, we report a novel ZooMS peptide marker that reliably distinguishes between horses and donkeys using the enzyme chymotrypsin. We apply this peptide marker to taxonomically identify bones from the Iberian Peninsula ranging from the Iron Age to the Late Modern Period. The peptide biomarker has the potential to facilitate the collection of morphological data for zooarchaeological studies of equids in Iberia and throughout Eurasia and Africa. • Novel ZooMS biomarker discriminates between horses and donkeys. • Novel biomarker provided species level identification for archaeological samples identified only to Equus. • The enzyme, chymotrypsin, reliably provides unique peptide markers for ZooMS analysis. [ABSTRACT FROM AUTHOR]

• The archaeology of climate change must. • Improve chronological controls on archaeological and environmental data; • Downscale climate data to local and microlocal effects in high-resolution studies; • Generate multi-proxy archaeological data analyses, avoiding single variable reporting in favor of multiple sources of precisely dated archaeological evidence; • Engage with up-to-date high-quality theory on social vulnerability and human responses to climate and environmental change; • Specify the threats, magnitude, and predictability of climate impacts in the context of traditional environmental knowledge; and. • Look at change before, during and after the climate impact. In 2004, the Journal of Anthropological Archaeology published Julie Field's "Environmental and climatic considerations: a hypothesis for conflict and the emergence of social complexity in Fijian prehistory", where she combined climate and environmental data to investigate the relationship between social patterns of change in the context of variability. Field tackled a complex issue: how societies respond to climate and environmental change that could cause unpredictable impacts on subsistence and settlement systems. Much progress has been made towards the understanding of human adaptations or responses to climate change since that paper was published. Here I reflect on the issues put forth by Field and consider how archaeology can engage with other interdisciplinary sciences and contribute to the understanding of human responses to environmental change. Two topics are emphasized: climate and environmental change, and social behavior in the context of that change. When attempting to identify how climate change affects a society, it is necessary to downscale climate to the specific location in consideration (space) during the time-period during which the relevant society lived (time) and in the context of their past experiences of climate (traditional knowledge). We face an unprecedented climate crisis and archaeology can contribute to the identification of solutions. [ABSTRACT FROM AUTHOR]

In a reply to our paper presenting new evidence supporting an intentional Neanderthal burial at La Chapelle-aux-Saints (Corrèze, France), Dibble et al. (2015) reviewed our data in relation to the original Bouyssonie publications. They conclude that alternative hypotheses can account for the preservation of the human remains within a pit. Here we present new data from our recent excavations and highlight several misinterpretations of the Bouyssonie publications, which, when taken together refute most of their arguments. Moreover, we show that the different hypotheses proposed by Dibble et al. cannot work together and fail to provide a credible explanation for the deposit, reinforcing our demonstration that the burial hypothesis remains the most parsimonious explanation for the preservation of the Neanderthal skeletal material at La Chapelle-aux-Saints. [ABSTRACT FROM AUTHOR]

During the course of long-term excavations of El Mirón Cave in Cantabrian Spain, remains of an adult human woman were found in deposits dating to the Lower Magdalenian (18.9–18.7 cal. kya). Interred with abundant red ochre (including specular hematite crystals) in culturally rich sediments characterized by abundant lithic and osseous artifact assemblages, faunal remains dominated by red deer and ibex, with some marine shells from an Oldest Dryas shore ca. 25 km distant, the “Red Lady of El Mirón” was buried between the rear cave vestibule wall and a large block, both of which (but especially the latter-also stained with red ochre in proximity to the corpse) bear engravings, possibly symbolically related to the burial. The papers of this special issue of Journal of Archaeological Science present the stratigraphic and archeological contexts, environmental background, dating, taphonomy, spatial distribution, human osteology, dietary information of the skeleton, and the rock art, ochre, artifacts and faunas associated with this burial, the first major human interment of Magdalenian age to be discovered on the Iberian Peninsula. [ABSTRACT FROM AUTHOR]

This paper presents a preliminary summary of lithic and osseous artifacts recovered from El Mirón Level 504, whose ochre-stained sediments were associated with a Lower Magdalenian human burial. The lithic artifacts were concentrated to the south of the human remains. Debris indicate that manufacture was focused on late-stage bladelet reduction, which followed preparatory flake or blade removals. The lithic industry is rich in bladelet tools and other Lower Magdalenian diagnostic artifacts that were made on very high-quality flints. Osseous artifacts—principally antler sagaies —are highly fragmented, but their attributes are consistent with others known from El Mirón. Lithic and osseous artifacts are typical of the Lower Magdalenian of Cantabria. These artifacts were recovered from a level with significant microstratigraphic variation associated with cultural and natural formation processes, leaving unknown how extensively these materials were spatially and/or temporally displaced from their primary discard locations as part of Level 504 deposition. Interpreted as a single unit and compared with those from the underlying Level 505, these burial area materials testify to spatial continuity with the similarly rich Lower Magdalenian occupations identified in the El Mirón mid- and outer-vestibule into the vestibule rear. The artifacts found in Level 504 provide additional evidence that confirm the site's importance as a major Lower Magdalenian residential site in the montane zone of the Cantabrian region. [ABSTRACT FROM AUTHOR]

Abstract: Bioarchaeological research must balance scholarly commitment to the generation of new knowledge, descendants' interests in their collective past, and the now common practice of rapid re-interment of excavated human remains. This paper documents the first results of a negotiated protocol built on the retention of one tooth per archaeologically derived skeleton, teeth that can then be used for destructive testing associated with ancient DNA and stable isotope investigations. Seven archaeological sites dating from the 13th to 16th centuries provided 53 teeth, 10 of which were subdivided between DNA and isotope labs. All tooth roots yielded haplogroup results, and five provided more detailed sequence results. Stable isotopes of carbon and nitrogen document heavy reliance on maize among all individuals, as well as reliance on a diverse range of fish. This work establishes baseline mtDNA information for Northern Iroquoians, and confirms the value of using dental tissues for dietary reconstruction. Particularly when human remains are fragmentary or co-mingled, this approach holds promise for ongoing incorporation of bioarchaeology into reconstructions of past peoples' lives. [Copyright &y& Elsevier]

Abstract: Anthropologists require methods for accurately estimating stature and body mass from the human skeleton. Age-structured, generalized Least Squares (LS) regression formulas have been developed to predict stature from femoral length and to predict body mass in immature human remains using the width of the distal metaphysis, midshaft femoral geometry (J), and femoral head diameter. This paper tests the hypothesis that panel regression is an appropriate statistical method for regression modeling of longitudinal growth data, with longitudinal and cross-sectional effects on variance. Reference data were derived from the Denver Growth Study; panel regression was used to create one formula for estimating stature (for individuals 0.5–11.5 years old); two formulas for estimating body mass from the femur in infants and children (0.5–12.5 years old); and one formula for estimating body mass from the femoral head in older subadults (7–17.5 years old). The formulas were applied to an independent target sample of cadavers from Franklin County, Ohio and a large sample of immature individuals from diverse global populations. Results indicate panel regression formulas accurately estimate stature and body mass in immature skeletons, without reference to an independent estimate for age at death. Thus, using panel regression formulas to estimate stature and body mass in forensic and archaeological specimens may reduce second stage errors associated with inaccurate age estimates. [Copyright &y& Elsevier]

Abstract: On the basis of stable carbon and nitrogen isotope analysis of human remains, this paper provides evidence for the retention of hunter–gatherer economies among coastal inhabitants in northern Chile during the late Holocene – at the same time that inland populations were adopting agricultural economies. Coastal diets from the Caleta Vitor region of the Atacama Desert were dominated by marine-based foods, predominantly from upper trophic levels. The focus on reliable marine food resources is interpreted as a risk minimisation strategy in this marginal arid environment. Although these coastal hunter–gatherers adopted other goods and traditions from agricultural populations, their participation in this larger interregional exchange network did not affect their basic subsistence economies. Associated new radiocarbon dates from the site are also discussed. Skeletal remains and artefacts associated with the Caleta Vitor shell middens generally originate from sites with dates ranging from c. 9000–476 BP. However, the human remains that are the subject of this research cover the time period c. 4000–476 BP. [Copyright &y& Elsevier]

Abstract: In 2009, an archaeological intervention in the Valle da Gafaria (Lagos, Portugal) allowed the excavation of part of a leprosarium and an associated necropolis (15th–17th centuries). The individuals recovered were buried directly in the soil, in positions and orientations discordant to the prevailing Christian rules. The sample is made up of eleven adult individuals of both sexes. This paper discusses the differential diagnosis of unusual and distinct pathological changes in five of the individuals recovered. The differential diagnosis of the lesions gave rise to several possible pathological conditions, namely, leprosy, treponematosis, brucellosis, slipped femoral capital epiphysis, and Legg-Calvé-Perthes disease. Various macroscopic and radiological aspects led us to consider leprosy as the most probable diagnosis in two individuals. Treponematosis and brucellosis were the probable diagnosis in other two individuals, respectively. One individual presented lesions in the right femur compatible with Legg-Calvé-Perthes disease. Historical documents suggest that the poor, mentally disabled, and people suffering from syphilis, tuberculosis, among others, were also housed in leprosaria. Therefore, it is possible that evidence of other conditions is found in human remains uncovered at a leprosarium. The cases under study enrich the scanty osteoarchaeological documentation of palaeopathology in Portugal in the Modern Age, namely of leprosy, brucellosis, treponematosis, and Legg-Calvé-Perthes disease, and they can contribute to reduce the discrepancy between historical and biological evidences of disease. [Copyright &y& Elsevier]

Abstract: In this paper we use Scanning and Transmission Electron Microscopy, and Energy Dispersive Spectroscopy to characterize the surface of the skin of a 2300 YO, naturally-preserved mummy, belonging to a two-year and eight month girl (“Pepita”), found inside a cave located in Altamira, Querétaro, México (21°40′–20°01′; 99°03′–100°36′). The cave was found in Sierra Gorda, an orographic region with a relief of sedimentary origin from the Gulf of Mexico, composed by high mountains with altitude values surpassing 3000 m above sea level, with ample and steep canyons, and a prominent role on the exploitation and distribution of cinnabar (HgS). The skin showed the presence of small-sized spherules, containing Al (≤43%) in the most exposed region (5–10 μm depth). Thin layers and structural microdomains covered small and large spheres. Structures conformed by stacked, nano-sized particles located far-from-the bunches contained C (≤45%), Zn and Si (≤10%), and minor amounts of Ca (≤2.6%). By contrast, regions between spheres contained high amounts of Ca (≤23%) and Al (≤15%), but lacked Zn and Si. Carbon spheres showed two distinctive composition, a signature that their formation might have occurred in a least two different stages via concentric growth mechanisms, with the incorporation of Zn and Al at a later stage. [Zn][Al]Carbon spheres showed morphology and growth patterns that compared to those resulting from the hydrothermal carbonization by Fe2+ ions under mild conditions, suggesting a common mechanism of formation. Textural changes of thin films found between [Zn][Al]Carbon spheres were attributed to differences in viscosity, which might have contributed to increases in functionality and specific surface area (by means of decreases in size) and, in turn, facilitating the sequestration of biomolecules. We propose that the presence of [Zn][Al]Carbon spheres provides protection against bacterial and UV attack. The physical properties of these spheres helped entrap biomolecules. Taken together, these factors contributed to the preservation of Pepita. [Copyright &y& Elsevier]

Abstract: This paper presents the results of stable isotope (carbon and nitrogen) analysis of human and faunal remains from the site of Aktopraklık, one of the earliest farming sites in the Eastern Marmara region of Northwest Anatolia. Excavations at this site have shown that occupation occurs from the middle of the 7th millennium BC through to the middle of the 6th millennium BC. The earliest Neolithic activity at this location occurs at the settlement site of Aktopraklık C. Since 2004 a number of Neolithic and Early Chalcolithic burials have been recovered from the settlement areas and an Early Chalcolithic cemetery (Aktopraklık B and A respectively). To date a total of 60 individuals have been recovered from Aktopraklık, 23 of which (20 adults [10 males, 8 females and 2 indet adults] and 3 children below ca. 12 years of age) form the basis of the current isotope study. In addition, 14 faunal samples from cattle, pig, sheep/goat and fallow deer are included in the analysis in order to facilitate a consideration of trophic level shifts and to interpret the 13C data. The data represents the first isotopic study of a farming community from this region of Anatolia. This region is important to our understanding of the north-westwards transmission of farming into Europe from the Near East, and as such Aktopraklık represents a key site for studying the diet of farmers at the transition to agriculture. The close clustering of isotope values overall indicates homogeneity in subsistence practices for this farming population. Interestingly, the isotope values indicate a general focus on C3 terrestrial resources at Aktopraklık, despite the close proximity of both freshwater and marine environments where alternative resources could have been procured. [Copyright &y& Elsevier]

Abstract: In order to investigate how the population diversity at major Romano-British urban centres compared to small towns and military outposts, we conducted multi-isotope (carbon, nitrogen, oxygen and strontium) analyses of bones (42 individuals) and teeth (26 individuals) of human skeletons from Cataractonium/Roman Catterick in North Yorkshire (U.K.). The results suggest a markedly less diverse population at Catterick than at the larger towns. Significant differences are observed between burials from the town and fort area and the suburb of Bainesse to the south, and it is suggested that these reflect a shift to more localised recruitment for the Roman army in the Late Roman period. Isotope data for the ‘Bainesse Eunuch’, an unusual 4th century burial that has been interpreted as the remains of a ‘transvestite’ priest of Cybele, are not ultimately conclusive but consistent with origins in Southern Britain or areas with a similar climate abroad. This paper also presents strontium isotope data for modern vegetation samples from 17 sites in the Catterick/northern Vale of York area which contribute to a continuing effort to map the biosphere 87Sr/86Sr variation in Britain. [Copyright &y& Elsevier]

Abstract: Strontium isotope ratio (87Sr/86Sr) analysis of archaeological human skeletal remains is an efficient method of investigating past population movement and residential mobility by determining probable geographical origins for the individuals examined. For this to be achieved, however, a map of biologically available 87Sr/86Sr signatures across the region investigated is needed. This paper presents a first such map for the Aegean, based on 87Sr/86Sr values recovered mainly from archaeological animal dental enamel and modern snail shells from sites largely distributed in the southern part of this region. Although not exhaustive, this comprehensive dataset of local biologically available 87Sr/86Sr signatures shows a marked difference between Mainland sites of the Pindos and Parnassos zones and the islands of south-eastern Aegean crossed by the Sub-Pelagonian zone (0.70808–0.70869), and sites in the central Cyclades and the north-eastern Aegean islands falling into the Attic-Cycladic metamorphic belt and the Vardar zone, respectively (0.70926–0.71187). Biologically available 87Sr/86Sr values from sites on central Euboea in the Pelagonian zone, south-eastern Attica and the western Cyclades in the Attic-Cycladic metamorphic belt, and on Crete in the Gavrovo zone are also relatively high (0.70853–0.70931), but lower than those recorded for the central Cyclades and the north-eastern Aegean. [Copyright &y& Elsevier]

Abstract: Excavations at Liang Bua, on the Indonesian island of Flores, have yielded a stratified sequence of stone artifacts and faunal remains spanning the last 95k.yr., which includes the skeletal remains of two human species, Homo sapiens in the Holocene and Homo floresiensis in the Pleistocene. This paper summarizes and focuses on some of the evidence for Homo floresiensis in context, as presented in this Special Issue edition of the Journal of Human Evolution and elsewhere. Attempts to dismiss the Pleistocene hominins (and the type specimen LB1 in particular) as pathological pygmy humans are not compatible with detailed analyses of the skull, teeth, brain endocast, and postcranium. We initially concluded that H. floresiensis may have evolved by insular dwarfing of a larger-bodied hominin species over 880k.yr. or more. However, recovery of additional specimens and the numerous primitive morphological traits seen throughout the skeleton suggest instead that it is more likely to be a late representative of a small-bodied lineage that exited Africa before the emergence of Homo erectus sensu lato. Homo floresiensis is clearly not an australopithecine, but does retain many aspects of anatomy (and perhaps behavior) that are probably plesiomorphic for the genus Homo. We also discuss some of the other implications of this tiny, endemic species for early hominin dispersal and evolution (e.g., for the “Out of Africa 1” paradigm and more specifically for colonizing Southeast Asia), and we present options for future research in the region. [Copyright &y& Elsevier]

Abstract: Excavations at Liang Bua, Flores, Indonesia, have yielded evidence for an endemic human species, Homo floresiensis, a population that occupied the cave between ∼95–17ka. This discovery has major implications for early hominin evolution and dispersal in Africa and Asia, attracting worldwide interest. This preface describes the rationale for the excavations in historical, geographical, and wider research contexts, as well as the methods used. It also introduces the other papers on aspects of Liang Bua research that feature in this edition of the Journal of Human Evolution. [Copyright &y& Elsevier]

Abstract: An extraordinarily well-preserved skeleton of a child, interred in a stone sarcophagus in the Late-Roman era, was discovered in the city of Mainz (Germany) in 1998, covered with a puff pastry-like substance assumed to be adipocere. It is the first time that this substance, which is derived from fat under oxygen-deficient conditions and prevents corpses from decaying, has been discovered on corpses buried under conditions described in the present paper. The body was buried at groundwater level (2.9m below surface) in a moist zone close to the Rhine that was affected by seasonally fluctuating groundwater levels. The fluctuating groundwater levels would appear to have had an effect on the degradation of the interred body. The discovery of the skeleton gave us an excellent opportunity to examine fatty acid material which had been subjected to prolonged fluctuating aerobic and anaerobic conditions in a moderate environment. The body''s fatty acid composition and 13C abundance were determined and compared with modern adipocere values. Element analysis of the stone sarcophagus in which the child was buried provided information on the burial environment. Our findings indicate that fatty material must have been converted into adipocere under anaerobic conditions in periods of high water levels, leaving the material open to decay during periods of low water levels. The fact that the excavated body was still covered with adipocere 1600 years after the burial clearly shows the robustness of the material against decay. [Copyright &y& Elsevier]

Abstract: Skeletal remains of Pazyryk warriors unearthed in a recent archaeological excavation in the Mongolian Altai offer a unique opportunity for verifying ancient histories of warfare and violence given by Herodotus in the fifth century BC. The Pazyryks were Iron Age nomadic groups associated with the eastern Scythians and known from burial site discoveries on the high steppes of the Altai (Central Asia). The aim of this paper is to analyze the evidence for bone trauma provided by the skeletal remains of these Pazyryk warriors with a particular focus on violence-related injuries. The sample consists of 10 individuals, comprising seven adult males, one adult female and two children. Seven individuals exhibited a total of 14 traumatic injuries. Six of these injuries (43%) showed evidence of bone remodelling and eight injuries (57%) were morphologically compatible with a perimortem origin. Twelve injuries (86%) were related to interpersonal violence, most likely caused by weapons similar to those found in Pazyryk tombs (battle-axes, daggers and arrowheads). Five individuals, including the female and one child, exhibited evidence of violent death. Furthermore, one individual also exhibited evidence of scalping. Despite the small number of Pazyryk skeletons analyzed, the pattern of traumatic injuries observed appears to be in agreement with that documented in conflicts related to raids or surprise attacks, and not a result of routinized or ritualized violence. These findings contribute new data to osteological evidence from Scythian burial sites. [Copyright &y& Elsevier]

Abstract: The extent of racemization of aspartic acid (Asp) – expressed as d/l ratio – has been used as a marker of biomolecular degradation in ancient remains. However, Asp racemization rate is highly variable, and depends on biochemical and geochemical factors. In this paper we aim to determine to which extent the fraction analyzed and the kind of sample used may influence the d/l Asp ratios. Other factors, such as burial site and sample preservation conditions, are also considered. d/l Asp ratios were obtained in 38 ancient human samples from 30 different individuals in which Real Time PCR quantification and amplification of short mtDNA fragments had been previously achieved. Four samples were taken from bones, 16 from whole teeth and 18 from dentine. In 7 cases whole tooth and dentine fractions from the same individual were analyzed. The samples belonged to 8 archaeological sites from Pre-pottery Neolithic B (PPNB), Cardial Neolithic and Chalcolithic time periods. Results show significant differences between the d/l Asp ratios obtained in dentine and whole tooth fractions from the same tooth and individual, as well as among dentine samples from the same archaeological site and among samples of the same age from different, though nearby, archaeological sites. Ancient DNA (aDNA) could be characterized in the majority of the samples, independently from their racemization values, which suggests that other factors, apart from the sample preservation stage, are more significantly affecting the racemization rate. A generalized use of the Asp racemization ratio as a threshold value for ancient DNA preservation needs to be questioned until further methodological standardization is considered. [Copyright &y& Elsevier]

Abstract: The context of burials in archaeological sites, that is whether the body was inhumed, wrapped, or in a coffin, is an aspect of mortuary ritual that has been missing from English-language publications on the subject. This is despite the development and use in France over at least the last two decades of methods of determining the context under the rubric ‘l''Anthropologie de Terrain’, or Field Anthropology. This paper briefly reviews the methods and applies them to prehistoric burial samples from two sites in Southeast Asia. This shows that burials at the Bronze Age site of Ban Lum Khao were either in coffins or wrapped. The practice of coffin burial appears to have been abandoned later, as all burials at the nearby Iron Age site of Noen U-Loke were wrapped. [Copyright &y& Elsevier]

Abstract: This paper describes the multi-disciplinary approach to reconstruct the face of Dante Alighieri (1265–1321). Since Dante''s sepulchre will be opened in 2021, the reconstructive process is based on morphological and metric data collected on the poet''s cranium during the formal identification of his remains in 1921 by the anthropologist Fabio Frassetto, as well as on the resulting plaster model. Starting from this plaster model and a morphologically compatible reference mandible, since the original mandible was never found, a 3D digital model of the complete skull was obtained by reverse engineering and virtual modelling techniques. The most important aspect in this work was the method of virtual modelling proposed for the ex novo generation of the mandible. The physical model of the skull (cranium+mandible) was then produced by means of a rapid prototyping system. This model was finally used to recreate Dante''s face via traditional facial reconstruction techniques currently used in forensic anthropology. [Copyright &y& Elsevier]

Abstract: This paper presents preliminary results of new radiocarbon dating of human, faunal and fish skeletal remains from a number of the cemeteries from the Middle and Lower Dnieper Basin, Ukraine. The results appear to demonstrate the presence of a radiocarbon reservoir effect during the Neolithic–Eneolithic periods, the first time that this has been identified in this region. The data indicate that the radiocarbon reservoir effect is currently only evident during these periods, and not in the earlier Epipalaeolithic–Mesolithic periods, prompting the suggestion that the radiocarbon reservoir effect is associated with elevated fish consumption from the Neolithic period onwards. [Copyright &y& Elsevier]

Abstract: The “Red Lady” partial human skeleton found at Goat''s Hole, Paviland, in south Wales by William Buckland in 1823 is one of the iconic relics of the British Paleolithic. Originally thought to be Roman, a Paleolithic age has been suspected from the middle of the 19th century. Several attempts have been made at directly radiocarbon dating the “Red Lady,” and here we report new determinations that suggest that it is, by a significant margin, the oldest of a group of ‘rich,’ Mid-Upper Paleolithic burials. We list similar Gravettian-aged burials from Europe, which have been dated recently for comparison. In this paper, we also reconsider the chronology of human use of the cave, apart from as a burial location. [Copyright &y& Elsevier]

Abstract: The paper explores the seldom-admitted fact that there are very few, if any, perfect osteological characters differentiating closely related species of similar size. Most characters described in various guide books to the identification of skeletal remains turn out to be less than perfect if a longer series of specimens is checked. However, the atypical or intermediate specimens that are sometimes found do not necessarily mean that the character is useless. It is suggested that from the ethical point of view it is acceptable to describe less than perfect characters provided that the cut-off frequency is explicitly stated. [Copyright &y& Elsevier]

Abstract: In this paper, three approaches for developing sample-specific sex determination methods of immature skeletal remains based on permanent tooth dimensions are proposed and tested using a sample of identified skeletons. The sample comprises adult and subadult individuals selected from the Lisbon documented skeletal collection, housed at the National Museum of Natural History in Lisbon, Portugal. Faciolingual and mesiodistal diameters were the tooth dimensions utilized. In the first approach, sex-specific logistic regression formulae based on adult tooth dimensions are developed and then used to determine the sex of the subadult sample. The second and third approaches are based on the sectioning point procedure, which uses the overall mean of a measurement (tooth diameter) collected from the sample as the discriminant criteria for determining the sex of the individuals in that sample. While in the second approach the adult overall mean of each dimension is used as the discriminant criteria for determining the sex of the subadults, in the third approach it is the subadult overall mean of each dimension that comprises the discriminant criteria that is applied back to the subadults for determining sex. Results show that the canines are the teeth with the highest sexual dimorphism and methods of sex determination based on canine dimensions provide correct allocation accuracies between 58.8% and 100% depending on the diameter and the approach that is being used. Canine faciolingual dimensions provide the best overall results. Combinations of measurements from the same and different teeth do not increase significantly the accuracy of the methods and approaches. Some of the problems of subadult sex determination methods based on adult tooth dimensions result from differing levels of sexual dimorphism between the adult and subadult segment of the sample. Mortality or cultural bias may increase or decrease the sexual dimorphism of the subadults compared to the adults. Small subadult samples utilized in this study may also raise questions regarding the accuracy of the three different sample-specific approaches. However, high consistency of results using the canine and different approaches, suggests that adult and subadult canine dimensions can be reliable sex discriminators of immature skeletal remains in archaeological samples. The major advantage of the approaches presented here is that they can be used to derive sample-specific methods and, therefore, eliminate the problem of applying morphological or metric methods to individuals originating from a population that differs from the one that contributed to the development of the method. [Copyright &y& Elsevier]

Abstract: This paper reports the δ 13C and δ 15N values of bone collagen, muscle and skin from several late prehistoric–early colonial (AD 1490–1640) mummies from Perú''s Ayacucho Valley. The mean of the δ 13C and δ 15N values of bone collagen are −11.5±1.4 and 11.1±0.7‰, respectively. The mean of the δ 13C values for Vinchos skin is −11.8±1.2‰ and the mean of δ 15N values is 13.2±0.5‰. The samples of muscle tissue have a δ 13C mean of −11.9±0.9‰ and a δ 15N mean of 12.7±0.3‰. The data from bone collagen indicate maize was the basis of the region''s subsistence economy. A significant correlation between δ 13C and δ 15N values of bone collagen (R 2 =0.75) is consistent with the preferential fertilization of maize with composted manure. Both skin and muscle samples are consistently enriched in δ 15N relative to paired samples of bone (2.1±0.5 and 1.6±0.7‰, respectively), possibly as a result of short term physiological stress or differential decomposition. [Copyright &y& Elsevier]

• Uncertain origin of modern artificial dental modification among Ngäbe indigenous group in Panama. • Surveyed teeth from pre- and post-contact archaeological sites. • Surveyed ethnohistorical literature of dental modification. • Hypothetical African origin of Ngäbe practice is upheld. Intentional dental modification is a widespread practice in both ancient and modern populations. In Panama, the modern practice is restricted to the Ngäbe indigenous people inhabiting the western provinces. Several researchers have posited that Ngäbe dental modification evidences cultural transfer of African origin due to the absence of post-contact records of this practice in the region, and based on the chipping technique used to create a pointed tooth shape. In this paper, we collate bioarchaeological data from human remains recovered from pre-contact and early colonial period contexts in Panama to evaluate this hypothesis. The results of our study found no evidence for intentional dental modification among the pre-contact sample, but several instances of artificially modified incisor teeth among the early colonial sample. The latter pertained exclusively to individuals of African ancestry, and whose teeth had been chipped to points in the same manner as reported from Ngäbe communities. Isotope data revealed that one individual was a first-generation immigrant who likely originated from the African continent. Based on these results, as well as an exhaustive review of the ethnohistorical and modern ethnographic literature, the original hypothesis of a late introduction of African origin for the practice of dental shaping among the Ngäbe was upheld. [ABSTRACT FROM AUTHOR]

Few European sites have yielded human dental remains safely dated to the end of MIS 4/beginning of MIS 3. One of those sites is Marillac (Southwestern France), a collapsed karstic cave where archeological excavations (1967–1980) conducted by B. Vandermeersch unearthed numerous faunal and human remains, as well as a few Mousterian Quina tools. The Marillac sinkhole was occasionally used by humans to process the carcasses of different prey, but there is no evidence for a residential use of the site, nor have any hearths been found. Rare carnivore bones were also discovered, demonstrating that the sinkhole was seasonally used, not only by Neanderthals, but also by predators across several millennia. The lithostratigraphic units containing the human remains were dated to ∼60 kyr. The fossils consisted of numerous fragments of skulls and jaws, isolated teeth and several post-cranial bones, many of them with traces of perimortem manipulations. For those already published, their morphological characteristics and chronostratigraphic context allowed their attribution to Neanderthals. This paper analyzes sixteen unpublished human teeth (fourteen permanent and two deciduous) by investigating the external morphology and metrical variation with respect to other Neanderthal remains and a sample from modern populations. We also investigate their enamel thickness distribution in 2D and 3D, the enamel-dentine junction morphology (using geometric morphometrics) of one molar and two premolars, the roots and the possible expression of taurodontism, as well as pathologies and developmental defects. The anterior tooth use and paramasticatory activities are also discussed. Morphological and structural alterations were found on several teeth, and interpreted in light of human behavior (tooth-pick) and carnivores' actions (partial digestion). The data are interpreted in the context of the available information for the Eurasian Neanderthals. [ABSTRACT FROM AUTHOR]