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Monitoring pig drinking has been a topic of interest to pig researchers and producers for many years. However, challenges still remain due to the fact that pigs like to play with drinkers in nursery environments and that the drinking pig is often touching others. These factors negatively influence the performance of camera-based pig drinking detection algorithms. The aim of this study is to investigate a deep learning method based on convolutional neural network (CNN) and long short-term memory (LSTM) to classify drinking and drinker-playing. In the experiment, two pens of pigs were video recorded for 3 days. In video from the first pen, 5400 2 s drinking episodes and 5400 2 s drinker-playing episodes were generated with 80% of these data being allocated as training set and the remaining 20% as validation set. In video from the second pen, 12,000 2 s drinking and drinker-playing episodes were generated as a test set. Firstly, the CNN architecture ResNet50 was used to extract spatial features. These features were input into LSTM framework to extract spatial–temporal features. Through the fully connected layer, the prediction function Softmax was finally used to classify these drinking and drinker-playing episodes. In the test set, the classification accuracy in the body and head regions of interest was 87.2% and 92.5%, respectively. The results indicate that the proposed method can be used to classify pigs' drinking and drinker-playing. These classification results have potential to improve the accuracy of pig drinking detection and help farmers to estimate pig welfare. • A novel video-based deep learning method was used to study pig drinking behaviour. • Long short-term memory was used to extract the spatial–temporal features. • Classification accuracy in the body and head regions of interest was 87.2% and 92.5%. • Drinking behaviour can be detected under conditions of pigs touching and overlapping. • Shortening the region of interest can be used to improve the classification accuracy. [ABSTRACT FROM AUTHOR]

Crotonylation on lysine sites in human non-histone proteins plays a crucial role in biology activities. However, because traditional experimental methods for crotonylation site identification are time-consuming and labor-intensive, computational prediction methods have become increasingly popular in recent years. Despite its significance, crotonylation site prediction has received less attention in non-histone proteins than in histones. In this study, we proposed a Multi-View Neural Network for identification of Human Non-Histone Crotonylation sites, named MVNN-HNHC. MVNN-HNHC integrated multi-view encoding features and adaptive encoding features through multi-channel neural network to deeply learn about attribute differences between crotonylation sites and non-crotonylation sites from various aspects. In MVNN-HNHC, convolutional neural networks can obtain local information from these features, and bidirectional long short term memory networks were utilized to extract sequence information. Then, we employ the attention mechanism to fuse the outputs of various feature extraction modules. Finally, the fully connection network acted as the classifier to predict whether a lysine site was crotonylation site or non-crotonylation site. Performance metrics on independent test set, including sensitivity, specificity, accuracy, Matthews correlation coefficient, and area under the curve (AUC) values reach 80.06 %, 75.77 %, 77.06 %, 0.5203, and 0.7792, respectively. To verify the effectiveness of this method, we carry out a series of experiments and the results show that MVNN-HNHC is an effective tool for predicting crotonylation sites in non-histone proteins. The data and code are available on https://github.com/xbbxhbc/junjun0612.git. [Display omitted] • In this study, we proposed a deep learning framework to identify non-histone crotonylation sites, integrating multi-view encoding features. • MVNN-HNHC applied multi-channel neural network for various types of features to deeply learn about discriminative attributes. • CNN, BLSTM are used to extract features and reduce dimensions, so as to obtain more valuable information. [ABSTRACT FROM AUTHOR]