1. Identification of differentially expressed genes in cucumber (Cucumis sativus L.) root under waterlogging stress by digital gene expression profile
- Author
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Qi, Xiao-Hua, Xu, Xue-Wen, Lin, Xiao-Jian, Zhang, Wen-Jie, and Chen, Xue-Hao
- Subjects
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GENE expression , *CUCUMBERS , *AMINO acid sequence , *PHOTOSYNTHESIS , *POLYMERASE chain reaction , *SUPEROXIDE dismutase , *TRANSCRIPTION factors , *REACTIVE oxygen species , *CARBON metabolism - Abstract
Abstract: High-throughput tag-sequencing (Tag-seq) analysis based on the Solexa Genome Analyzer platform was applied to analyze the gene expression profiling of cucumber plant at 5 time points over a 24h period of waterlogging treatment. Approximately 5.8million total clean sequence tags per library were obtained with 143013 distinct clean tag sequences. Approximately 23.69%–29.61% of the distinct clean tags were mapped unambiguously to the unigene database, and 53.78%–60.66% of the distinct clean tags were mapped to the cucumber genome database. Analysis of the differentially expressed genes revealed that most of the genes were down-regulated in the waterlogging stages, and the differentially expressed genes mainly linked to carbon metabolism, photosynthesis, reactive oxygen species generation/scavenging, and hormone synthesis/signaling. Finally, quantitative real-time polymerase chain reaction using nine genes independently verified the tag-mapped results. This present study reveals the comprehensive mechanisms of waterlogging-responsive transcription in cucumber. [Copyright &y& Elsevier]
- Published
- 2012
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