1. ERK binds, phosphorylates InsP3 type 1 receptor and regulates intracellular calcium dynamics in DT40 cells
- Author
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Yang, Ling-Hai, Bai, Gui-Rong, Huang, Xiu-Ying, and Sun, Fang-Zhen
- Subjects
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PHOSPHORYLATION , *CHEMICAL reactions , *VITAMIN B complex , *AMINO acids - Abstract
Abstract: Modulation on the duration of intracellular Ca2+ transients is essential for B-cell activation. We have previously shown that extracellular-signal-regulated kinase (ERK) can phosphorylate inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) at serine 436 and regulate its calcium channel activity. Here we investigate the potential physiological interaction between ERK and IP3R1 using chicken DT40 B-cell line in which different mutants are expressed. The interaction between ERK and IP3R1 is confirmed by co-immunoprecipitation and fluorescence resonance energy transfer (FRET) assays. This constitutive interaction is independent of either ERK kinase activation or IP3R1 phosphorylation status. Back phosphorylation analysis further shows that type 1 IP3R (IP3R1) is phosphorylated by ERK in anti-IgM-activated DT40 cells. Finally, our data show that the phosphorylation of Ser 436 in the IP3-binding domain of IP3R1 leads to less Ca2+ release from endoplasmic reticulum (ER) microsomes and accelerates the declining of calcium increase in DT40 cells in response to anti-IgM stimulation. [Copyright &y& Elsevier]
- Published
- 2006
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