1. Restoration of miR-143 reduces migration and proliferation of bladder cancer cells by regulating signaling pathways involved in EMT.
- Author
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Asghariazar, Vahid, Kadkhodayi, Mahtab, Mansoori, Behzad, Mohammadi, Ali, and Baradaran, Behzad
- Subjects
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CANCER cell proliferation , *CELLULAR signal transduction , *CELL communication , *CANCER cell migration , *VIMENTIN , *CELL migration - Abstract
MicroRNAs (miRNAs), a class of regulatory endogenous short RNAs, are involved in various biological functions by targeting the mRNA of multiple protein-coding genes and influencing their related signaling pathways. In this investigation, we upregulated microRNA-143 (miR-143) expression levels in bladder cancer (BC) EJ138 cells by pCMV-miR-143 vectors. The efficacy of transfection was verified by Flow cytometry. The influence of miR-143 overexpression on BC cells migration, proliferation, and apoptosis was detected using wound-healing assay, MTT assay, and DAPI and Annexin V/PI staining, respectively. The results demonstrated that upregulation of miR-143 in BC EJ138 cells leads to inhibited proliferation and migration. Also, restoration of miR-143 was negatively associated with the expression levels of metastatic, apoptotic, invasion, and EMT-related genes, including C-Myc, CXCR4, MDM2, Vimentin, Snail-1, and MMP-9, along with increased E-Cadherin and TP53 expression. Therefore, miR-143 may be considered a potential therapeutic target for BC. [Display omitted] • Restoration of miR-143 significantly reduces cell proliferation and migration of bladder cancer cells (EJ138). • miR-143 involves different major signaling pathways involved in EMT, such as AKT , ERK , MAPK , and NF-κB. • The expression level of C-myc , MMP-9 , Snail-1 , CXCR4 , MDM2, and Vimentin downregulated by replacement of mir-143. • The expression level of E-cadherin and TP53 is upregulated by restoration of mir-143. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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