1. Respiratory syncytial virus infection activates STAT signaling in human epithelial cells
- Author
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Kong, Xiaoyuan, San Juan, Homero, Kumar, Mukesh, Behera, Aruna K., Mohapatra, Alexander, Hellermann, Gary R., Mane, Srikant, Lockey, Richard F., and Mohapatra, Shyam S.
- Subjects
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ADULT respiratory distress syndrome , *EPITHELIAL cells - Abstract
Acute respiratory syncytial virus (RSV) infection causes airway inflammation and exacerbates asthma, but the mechanism of inflammation is poorly understood. The role of the STAT-signaling pathway in RSV infection in epithelial cells was examined in this study. DNA microarray analyses of RSV-infected human alveolar type II (A549) epithelial cells identified several genes whose expression was altered from −5.5 to +56.4-fold. Four of the highly expressed genes contained STAT-binding elements. In A549 and normal human bronchial epithelial cells (NHBE), RSV induced phosphorylation and nuclear translocation of STAT-1α that was abrogated when RSV attachment was blocked. Treatment with a JAK-2 inhibitor or transfection with dominant-negative STAT-1α blocked STAT-1α activation and RSV infection. RSV also activated STAT-3 and IL-6 specific antibodies blocked this activation. Thus, activation of the STAT-1α and STAT-3 pathways play a role in RSV infection. [Copyright &y& Elsevier]
- Published
- 2003
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