1. A novel mycovirus closely related to viruses in the genus Alphapartitivirus confers hypovirulence in the phytopathogenic fungus Rhizoctonia solani.
- Author
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Zheng, Li, Zhang, Meiling, Chen, Qiguang, Zhu, Minghai, and Zhou, Erxun
- Subjects
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FUNGAL viruses , *PHYTOPATHOGENIC fungi , *RHIZOCTONIA solani , *MICROBIAL virulence , *DOUBLE-stranded RNA , *RICE sheath blight , *CLADISTIC analysis - Abstract
Abstract: We report here the biological and molecular attributes of a novel dsRNA mycovirus designated Rhizoctonia solani partitivirus 2 (RsPV2) from strain GD-11 of R. solani AG-1 IA, the causal agent of rice sheath blight. The RsPV2 genome comprises two dsRNAs, each possessing a single ORF. Phylogenetic analyses indicated that this novel virus species RsPV2 showed a high sequence identity with the members of genus Alphapartitivirus in the family Partitiviridae, and formed a distinct clade distantly related to the other genera of Partitiviridae. Introduction of purified RsPV2 virus particles into protoplasts of a virus-free virulent strain GD-118 of R. solani AG-1 IA resulted in a derivative isogenic strain GD-118T with reduced mycelial growth and hypovirulence to rice leaves. Taken together, it is concluded that RsPV2 is a novel dsRNA virus belonging to Alphapartitivirus, with potential role in biological control of R. solani. [Copyright &y& Elsevier]
- Published
- 2014
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