1. Activation of vascular endothelial cells by IL-<f>1α</f> released by epithelial cells infected with respiratory syncytial virus
- Author
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Chang, Cheng-Hsien, Huang, Yan, and Anderson, Robert
- Subjects
- *
RESPIRATORY syncytial virus , *CELL communication - Abstract
Although pulmonary inflammation is a serious, sometimes life-threatening, consequence of respiratory syncytial virus (RSV) infection, the mechanisms involved are not well understood. Since the process of inflammation is initiated by a complex series of events including the activation of specific adhesion molecules on vascular endothelium, we searched for endothelial cell-activating factors released from RSV-infected epithelial cells. We demonstrate here that vascular endothelial cells exposed to culture supernatants from RSV-infected pulmonary epithelial A549 cells are activated to express increased cell surface ICAM-1, and to a lesser extent, VCAM-1 and E-selectin. IL-
1α was identified as the predominant endothelial cell-activating factor by pretreating epithelial cell supernatants with anti-IL-1α antibody. The preferential upregulation of endothelial ICAM-1 (relative to VCAM-1 and E-selectin) by RSV-infected epithelial cell supernatants was replicated by recombinant IL-1α thus confirming IL-1α as a major endothelial cell-activating cytokine released by RSV-infected epithelial cells. Il-1α mediated endothelial cell activation is thus a likely contributory event in the initiation of leukocyte inflammation associated with RSV infection. [Copyright &y& Elsevier]- Published
- 2003
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