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Abstract: Eleven red wines imported from foreign country and 40 domestic fruit wines, including 15 red wines, 4 white wines, 7 plum wines, and 14 other fruit wines, sold in the supermarkets in Taiwan were purchased and tested to determine the occurrence of biogenic amines and histamine-forming bacteria. The levels of pH, total soluble solids (TSS), titratable acidity (TA), reducing sugar (RS), total sugar (TS), sulphites, methanol (milligram per liter of pure ethanol), ethanol and Pb in all samples ranged from 3.0 to 4.1, 6.8 to 24.4 °Brix, 0.3 to 1.7g/100mL, 0.2 to 17.6g/100mL, 1.6 to 28.4g/100mL, <2 to 260.5mg/L, <1 to 2559mg/L, 5.0 to 15.6g/100mL and <1 to 46.2μg/L, respectively. The levels of TSS, TA, RS, and TS in plum wine samples were significantly higher than those of the other wines samples, whereas the pH value in plum wine samples was lower than that of the other wines samples. The average content for each of the nine biogenic amines in all samples was less than 5.2mg/L. However, higher levels of histamine and spermine were detected in domestic fruit wine samples than the imported red wine samples. Five histamine-forming isolates isolated from domestic red wine and jackfruit wine, capable of producing 13.0mg/L to 69.1mg/L of histamine in trypticase soy broth (TSB) supplemented with 2g/100mL l-histidine (TSBH) or MRS broth supplemented with 2g/100mL l-histidine (MRSH), were identified as Bacillus pumilus (one strain), Bacillus sp. (two strains) and Acetobacter pasteurianus (one strain) by 16S rDNA sequencing with PCR amplification, and Zygoascus hellenicus var. hellenicus (one strain) by internal transcribed spacer sequencing with PCR amplification. To our knowledge, this is the first report to demonstrate the occurrence of histamine-forming bacilli bacteria, acetic bacteria and yeast in fruit wine. [Copyright &y& Elsevier]

Abstract: Objective: It is not clear whether the prognostic value of pretreatment serum CA125 levels is independent or through association with other clinicopathological features in endometrial cancer. Methods: All patients with endometrial cancer treated between 2000 and 2010 were retrospectively reviewed. The correlation of clinicopathological characteristics, CA125 and treatment outcomes was analyzed. Receiver operating characteristics (ROC) curves were used to determine the CA125 cut-off values. Cox proportional hazard regression was used for multivariate analysis. Results: Of the 923 eligible patients, 757 had serum CA125 levels measured before treatment. We identified 264 (34.9%) patients with pretreatment serum CA125>35U/mL. By multivariate analysis, advanced stage (P =0.001), serous or clear cell carcinoma (P =0.008), positive peritoneal cytology (P =0.042), and lymph node metastases (P =0.004) were significant risk factors for cancer-specific survival (CSS), while serum CA125>35U/mL (P =0.067) was of borderline statistical significance. Using ROC curve stratified by age, we found that a serum CA125>35U/mL was significant for CSS (HR=2.34, 95% CI=1.04–5.29) among patients >49years old. After adjustment for confounding factors, serum CA125>105U/mL was significant (HR=6.03, 95% CI=1.19–30.63) in patients ≤49years old. Conclusions: These results suggest that an age-stratified cut-off level for CA125 (35U/mL in patients >49years old and 105U/mL in patients ≤49years old) can improve the prognostic stratification of patients with endometrial cancer. [Copyright &y& Elsevier]