1. Characterization of polysaccharide from Astragalus radix as the macrophage stimulator
- Author
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Zhao, Lu-Hang, Ma, Zhi-Xin, Zhu, Jie, Yu, Xiao-Hong, and Weng, Deng-Po
- Subjects
- *
POLYSACCHARIDES , *ASTRAGALUS (Plants) , *MACROPHAGES , *HOT water , *EXTRACTION techniques , *GEL permeation chromatography , *IMMUNOLOGICAL adjuvants - Abstract
Abstract: Astragalus polysaccharide (APS) was obtained by hot water extraction, alcohol precipitation, gel-permeation chromatography and ultrafiltration. Fluorescence material 2-aminoacridone (2-AMAC) labeled APS bind to macrophage in a time- dependent manner and the binding can be remarkably inhibited by APS. Furthermore, the effect of APS on RAW264.7 macrophage demonstrated APS increase the level of cytokines including TNF-α, GM-CSF and the production of NO. NF-κB protein levels are increased in response to APS. Blocking NF-κB with specific inhibitor resulted in decreased levels of NO and TNF-α. The results suggested that APS possess potent immunomodulatory activity by stimulating macrophage and could be used as an immunotherapeutic adjuvant. [Copyright &y& Elsevier]
- Published
- 2011
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