1. PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20
- Author
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Nicholson, Ian C., Mavrangelos, Christos, Bird, Daniel R.G., Bresatz-Atkins, Suzanne, Eastaff-Leung, Nicola G., Grose, Randall H., Gundsambuu, Batjargal, Hill, Danika, Millard, Debbrah J., Sadlon, Timothy J., To, Sarah, Zola, Heddy, Barry, Simon C., and Krumbiegel, Doreen
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GENE expression , *PEPTIDASE , *ENZYME inhibitors , *MONOCLONAL antibodies , *CHEMOKINES , *PHENOTYPES - Abstract
Abstract: The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites. [Copyright &y& Elsevier]
- Published
- 2012
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