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Stable isotope analysis (N and C) of fossil cereal grains is regularly explored as a means of obtaining insights to past crop growing conditions and agricultural practices. In this study, we assessed how several growth conditions can affect δ15N and δ13C values of modern cereal grain with the aim to help understand isotopic values from ancient cereal remains. We investigated the impact of fertilizer intensity, plant density, and soil type on cereal grain δ15N and δ13C values in a short-term field experiment using hulled and naked barley, bread wheat, emmer wheat, einkorn, spelt wheat, rye, and oat. We found the following key results: 1) while fertilizing had a significant effect on δ15N values in grain, the impact varied between species, and cereals grown in heavy clay had consistently higher δ15N values in grain compared to those grown in light sandy soil, and 2) the δ13C values were significantly different between cereal species, but the difference was very similar between the two experimental site-locations. These findings obtained from plants grown under known conditions contribute to better understand how natural conditions and anthropogenic activities effect crop isotopic data and is of relevance for isotopic research on archaeological crop remains. • Field experiment assessing effects of growth conditions to cereal grain δ15N and δ13C values. • Effect of fertilizer intensity, plant density and soil type on crop isotope values. • Isotope analysis reveal cereal species are affected differently by experimental factors. • Soil type and fertilizer plays a profound role on crop δ15N values. • Difference in δ13C values between cereals similar at locations with different soils. [ABSTRACT FROM AUTHOR]

Measurements of archaeological cereal grains has the potential to improve interpretations of archaeobotanical assemblages and to address a broad range of research questions related to agricultural practices in the past. However, understanding the role of the many factors that can influence the morphometrics of cereal grains is of importance to validate the application of morphometrics in archaeobotany. The aim of this study was to investigate the potential effects of growing conditions on cereal grain size using three experimental factors: site-location with different soil types, manure intensity and plant density. A short-term field experiment was conducted at two locations (Alnarp and Holma) in southern Sweden with hulled barley, naked barley, bread wheat, emmer wheat, einkorn, spelt wheat, rye and oat. Our results show that, while all three factors investigated affect grain size, most significant effect was from manuring (p < 0.0001) and site location with different soil types (p < 0.0001), plant density had the weakest effect (p = 0.005), and that cereal species reacted differently to experimental factors. We further use the morphometric dataset to assess the relationship between grain size variation and growing conditions, using the sample range of grain size from different experimental factors. The experimental data obtained sheds light on how growing conditions may affect grain size and the results are discussed in relation to grain size composition in archaeobotanical assemblages. • Experimental study show the effect of growing conditions on cereal grain size. • Manuring and site-location had a stronger influence on grain size compared to plant density. • Wide variation in grain size was observed within and between investigated growing conditions. • Observed sample ranges of grain sizes helps to better interpret archaeobotanical assemblages. [ABSTRACT FROM AUTHOR]

Patients at increased cardiovascular risk commonly display high levels of plasma triglycerides (TGs), elevated LDL cholesterol, small dense LDL particles and low levels of HDL-cholesterol. Many remain at high risk even after successful statin therapy, presumably because TG levels remain high. Lipoprotein lipase (LPL) maintains TG homeostasis in blood by hydrolysis of TG-rich lipoproteins. Efficient clearance of TGs is accompanied by increased levels of HDL-cholesterol and decreased levels of small dense LDL. Given the central role of LPL in lipid metabolism we sought to find small molecules that could increase LPL activity and serve as starting points for drug development efforts against cardiovascular disease. Using a small molecule screening approach we have identified small molecules that can protect LPL from inactivation by the controller protein angiopoietin-like protein 4 during incubations in vitro. One of the selected compounds, 50F10, was directly shown to preserve the active homodimer structure of LPL, as demonstrated by heparin-Sepharose chromatography. On injection to hypertriglyceridemic apolipoprotein A-V deficient mice the compound ameliorated the postprandial response after an olive oil gavage. This is a potential lead compound for the development of drugs that could reduce the residual risk associated with elevated plasma TGs in dyslipidemia. [ABSTRACT FROM AUTHOR]