1. Evidence that Moderate Eviction of Spt5 and Promotion of Error-Free Transcriptional Bypass by Rad26 Facilitates Transcription Coupled Nucleotide Excision Repair.
- Author
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Selvam, Kathiresan, Ding, Baojin, Sharma, Rahul, and Li, Shisheng
- Subjects
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RNA polymerase II , *DNA damage , *DNA polymerases , *EVICTION , *RNA polymerases , *SURGICAL excision - Abstract
Abstract Transcription coupled repair (TC-NER) is a subpathway of nucleotide excision repair triggered by stalling of RNA polymerase at DNA lesions. It has been suspected that transcriptional misincorporations of certain nucleotides opposite lesions that result in irreversible transcription stalling might be important for TC-NER. However, the spectra of nucleotide misincorporations opposite UV photoproducts and how they are implicated in transcriptional stalling and TC-NER in the cell remain unknown. Rad26, a low abundant yeast protein, and its human homolog CSB have been proposed to facilitate TC-NER in part by positioning and stabilizing stalling of RNA polymerase II (RNAPII) at DNA lesions. Here, we found that substantial AMPs but no other nucleotides are transcriptionally misincoporated and extended opposite UV photoproducts and adjacent bases in Saccharomyces cerevisiae. Rad26 does not significantly affect either the misincorporation or extension of AMPs. At normally low or moderately increased levels, Rad26 promotes error-free transcriptional bypass and TC-NER of UV photoproducts. However, Rad26 completely loses these functions when it is overexpressed to ~ 1/3 the level of RNAPII molecules. Also, Rad26 does not directly displace RNAPII but constitutively evicts Spt5, a key transcription elongation factor and TC-NER repressor, from the chromatin. Our results indicate that transcriptional nucleotide misincorporation is not implicated in TC-NER, and moderate eviction of Spt5 and promotion of error-free transcriptional bypass of DNA lesions by Rad26 facilitates TC-NER. Graphical Abstract Unlabelled Image Highlights • Rad26 was proposed to facilitate TC-NER in part by positioning and stabilizing RNAPII at DNA lesions. • Only AMPs are transcriptionally misincoporated and extended opposite UV photoproducts in the cell. • Only at low or moderate levels can Rad26 promote transcriptional bypass of UV photoproducts and TC-NER. • Rad26 does not directly displace RNAPII but evicts Spt5 from the chromatin. • Rad26 facilitates TC-NER by moderately evicting Spt5 and promoting transcriptional lesion bypass. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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