1. Fluorescent microsphere immunochromatographic sensor for the detection of total fumonisins B1, B2, and B3 in grain samples.
- Author
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Li, Xiaofang, Li, Jinyan, Feng, Yongwei, Liu, Liqiang, Kuang, Hua, Xu, Chuanlai, and Guo, Lingling
- Subjects
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FUMONISINS , *LIQUID chromatography-mass spectrometry , *CARCINOGENS , *MONOCLONAL antibodies , *REFERENCE sources - Abstract
Fumonisin Bs (FBs) are the most common toxic and carcinogenic substances found among naturally occurring contaminants of cereals. A group-specific monoclonal antibody (mAb) 2H7 was prepared that equably recognized fumonisins B 1 (FB 1), B 2 (FB 2), and B 3 (FB 3) with a half-maximal inhibitory concentration (IC 50) value for the three forms of 1.52, 2.93, and 3.85 ng/ml, respectively. On this basis, a fluorescent microsphere-based immunochromatographic strip (FM-ICS) was established for the rapid qualitative and quantitative detection of total FBs (FB 1 , FB 2 , and FB 3) in corn, wheat, and rice. A recovery test showed that the recovery of FM-ICS was 92.8–108.3% with coefficients of variation of 1.2–7.9%. This method has the same reliability as seen with liquid chromatography-tandem mass spectrometry. And the accuracy was further confirmed by certified reference materials. Therefore, it can be used for the detection of FBs in cereal samples. • A group-specific monoclonal antibody 2H7 was prepared that equably recognized fumonisins B 1 , B 2 , and B 3. • FM-ICS was established for the rapid qualitative and quantitative detection of total FBs in corn, wheat, and rice. • FBs certified reference materials confirmed the feasibility of FM-ICS. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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