1. Identification and functional analyses of CD4-1+ cells in grass carp (Ctenopharyngodon idella).
- Author
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Han, Xue-Qing, Pan, Yi-Ru, Zhong, Ya-Qin, Tian, Tian-Tian, Liu, Xun, Zhang, Xu-Jie, and Zhang, Yong-An
- Subjects
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CTENOPHARYNGODON idella , *CELL analysis , *MYELOID cells , *FUNCTIONAL analysis , *T cells - Abstract
In mammals, CD4 is found to be expressed on T cells and innate immune cells, however, teleost cells bearing CD4 have not been well identified and characterized. In this study, we identified two different CD4-1+ cell subsets in grass carp (Ctenopharyngodon idella): CD4-1+ lymphocytes (Lym) and CD4-1+ myeloid cells (Mye), both of which had the highest proportions in the head kidney. The mRNA expression analysis showed that CD4-1 , CD4-2 , TCRβ , CD3γ/δ , and LCK1 are highly expressed in CD4-1+ Lym and also expressed in CD4-1+ Mye. Furthermore, we found that CD4-1+ Lym have a Lym morphology and highly express T-cell cytokines, suggesting that they are CD4+ T cells equivalent to mammalian Th cells. On the other hand, CD4-1+ Mye were found to have a morphology of macrophage and highly express macrophage marker gene MCSFR , indicating that they are macrophages. In addition, functional analysis revealed that CD4-1+ Mye possess phagocytic ability and great antigen-processing ability. Taken together, our study sheds further light on the composition and function of CD4+ cells in teleost fish. • CD4-1+ lymphocytes and CD4-1+ myeloid cells were identified in grass carp. • CD4-1+ lymphocytes highly expressed T-cell-related genes and Th-cell cytokines. • CD4-1+ myeloid cells were found to highly express macrophage-related genes. • CD4-1+ myeloid cells exhibited phagocytic and antigen-processing abilities. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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