1. Higher PIK3C2B gene expression of H1N1+ specific B-cells is associated with lower H1N1 immunogenicity after trivalent influenza vaccination in HIV infected children.
- Author
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Cotugno, Nicola, Zicari, Sonia, Morrocchi, Elena, de Armas, Lesley R., Pallikkuth, Suresh, Rinaldi, Stefano, Ruggiero, Alessandra, Manno, Emma Concetta, Zangari, Paola, Chiriaco, Maria, Bernardi, Stefania, Andrews, Sarah F., Cagigi, Alberto, Rossi, Paolo, McDermott, Adrian B., Pahwa, Savita, and Palma, Paolo
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INFLUENZA vaccines , *B cells , *GENE expression , *IMMUNE response , *ANTIRETROVIRAL agents - Abstract
Perinatally HIV-infected children (PHIV), despite successful antiretroviral therapy, present suboptimal responses to vaccinations compared to healthy-controls (HC). Here we investigated phenotypic and transcriptional signatures of H1N1-specific B-cells (H1N1-Sp) in PHIV, differentially responding to trivalent-influenza-vaccine (TIV), and HC. Patients were categorized in responders (R) and non-responders (NR) according to hemagglutination-inhibition-assay at baseline and 21 days after TIV. No differences in H1N1-Sp frequencies were found between groups. H1N1-Sp transcriptional analysis revealed a distinct signature between PHIV and HC. NR presented higher PIK3C2B and NOD2 expression compared to R, confirmed by downregulation of PIK3C2B in resting-memory of R after H1N1 in-vitro stimulation. In conclusion this study confirms that qualitative rather than quantitative analyses are needed to characterize immune responses in PHIV. These results further suggest that higher PIK3C2B in H1N1-Sp of NR is associated with lower H1N1 immunogenicity and may be targeted by future modulating strategies to improve TIV responses in PHIV. Unlabelled Image • Similar frequency of H1N1-Sp was found between PHIV and HC regardless H1N1 response. • H1N1-Sp transcriptional signatures distinguished PHIV from HC. • Higher PIK3C2B and NOD2 gene expression of H1N1-Sp was revealed in PHIV NR to TIV. • PHIV NR to TIV failed to downregulate PIK3C2B after in vitro stimulation. • PIK3C2B pathway may play a critical role in immune response upon TIV in PHIV. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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