1. IGF-I enhances cellular senescence via the reactive oxygen species–p53 pathway
- Author
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Handayaningsih, Anastasia-Evi, Takahashi, Michiko, Fukuoka, Hidenori, Iguchi, Genzo, Nishizawa, Hitoshi, Yamamoto, Masaaki, Suda, Kentaro, and Takahashi, Yutaka
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SOMATOMEDIN C , *CELLULAR aging , *REACTIVE oxygen species , *P53 antioncogene , *GALACTOSIDASES , *CARCINOGENESIS - Abstract
Abstract: Cellular senescence is characterized by growth arrest, enlarged and flattened cell morphology, the expression of senescence-associated β-galactosidase (SA-β-gal), and by activation of tumor suppressor networks. Insulin-like growth factor-I (IGF-I) plays a critical role in cellular growth, proliferation, tumorigenesis, and regulation of aging. In the present study, we show that IGF-I enhances cellular senescence in mouse, rat, and human primary cells in the confluent state. IGF-I induced expression of a DNA damage marker, γH2AX, the increased levels of p53 and p21 proteins, and activated SA-β-gal. In the confluent state, an altered downstream signaling of IGF-I receptor was observed. Treatment with a reactive oxygen species (ROS) scavenger, N-acetylcystein (NAC) significantly suppressed induction of these markers, indicating that ROS are involved in the induction of cellular senescence by IGF-I. In p53-null mouse embryonic fibroblasts, the IGF-I-induced augmentation of SA-β-gal and p21 was inhibited, demonstrating that p53 is required for cellular senescence induced by IGF-I. Thus, these data reveal a novel pathway whereby IGF-I enhances cellular senescence in the ROS and p53-dependent manner and may explain the underlying mechanisms of IGF-I involvement in tumorigenesis and in regulation of aging. [Copyright &y& Elsevier]
- Published
- 2012
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