1. Activation of adenosine A2A receptor induced interleukin-23 mRNA expression in macrophages of ankylosing spondylitis patients.
- Author
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Akhtari, Maryam, Vojdanian, Mahdi, Javinani, Ali, Ashraf-Ganjouei, Amir, Jamshidi, Ahmadreza, and Mahmoudi, Mahdi
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ADENOSINES , *ANKYLOSING spondylitis , *INTERLEUKIN-23 , *MACROPHAGE colony-stimulating factor , *TUMOR necrosis factors , *PATHOLOGY , *MESSENGER RNA - Abstract
• A 2A AR agonist decreases TNF-α secretion by macrophages of AS patients. • A 2A AR activation increases IL23A mRNA expression by macrophages of AS patients. • A 2A AR activation did not have any influence on macrophages of healthy individuals. Ankylosing spondylitis (AS) is an auto-inflammatory debilitating disorder with a complex pathogenesis. The adenosinergic pathway is an immunologic regulating pathway with a potential role in AS pathophysiology. In the present study, we have aimed to investigate the influence of A 2A adenosine receptor (A 2A AR) activation on tumor necrosis factor-α (TNF-α) and interleukin-23 (IL-23) expression and secretion by monocyte-generated macrophages of AS patients. Whole-blood separated monocytes were extracted from 14 AS patients and 14 healthy controls. Macrophages were differentiated by macrophage colony-stimulating factor (M-CSF), and surface markers were confirmed by flow cytometer. Cells were treated with CGS-21680 as a known agonist of A 2A AR. Analysis of ADORA2A , TNFA , and IL23A gene expression was performed by SYBR green real-time PCR. The concentration of secreted cytokines was also measured by ELISA kits. Based on our analysis, CGS-21680 significantly decreased TNF-α secretion by monocyte-derived macrophages of AS patients. Moreover, A 2A AR agonist increased the IL23A mRNA expression level in monocyte-derived macrophages of AS patients considerably. Whereas, CGS-21680 did not have any influence on macrophages of healthy individuals. According to our results, it appears that A 2A AR activation can increase IL-23 secretion by monocyte-derived macrophages of AS patients. Although the TNF-α reducing effect of A 2A AR agonists can be a potential target in AS treatment, robust increasing of IL-23 should be considered as the undesirable effect of these agents. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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