1. The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors.
- Author
-
Chen, Xiaoyi, Moran Torres, Juan P., Jan Vonk, Peter, Damen, J. Mirjam A., Reiding, Karli R., Dijksterhuis, Jan, Lugones, Luis G., and Wösten, Han A.B.
- Subjects
- *
ASPERGILLUS niger , *TRANSCRIPTION factors , *PHENOTYPES , *FUNGAL cell walls , *GENE silencing , *LYSIS , *DIGESTIVE enzymes - Abstract
• Genes abaA , aslA , aslB , azf1, htfA , nosA , and srbA are involved in sporulation. • Genes aslA , aslB , azf1, htfA , nosA , and srbA are involved in cell wall integrity. • Genes aslA , aslB , azf1, htfA , nosA , and srbA are involved in protein secretion. • These genes explain, at least part of, the pleiotropic phenotype of Δ flbA. Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in Δ flbA. Here, seven of these genes (abaA , aslA , aslB , azf1, htfA , nosA , and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of Δ aslA and Δ aslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of Δ abaA, Δ aslA , Δ nosA, and Δ srbA , while biomass was higher in liquid shaken cultures of Δ aslA and Δ aslB. The Δ aslA and Δ htfA strains showed increased resistance to H 2 O 2 , while Δ aslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of Δ flbA of A. niger. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF