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Tea tree is a fluorine (F)-enriched plant, leading to much concern about the safety of drinking tea from tea tree (Camellia sinensis (L.) Kuntze). Tea tree is a perennial leaf-harvested crop, and tea production in China is generally categorized as spring tea, summer tea and autumn tea in its annual growth rounds. However, the seasonally dynamic changes of F content and accumulation in the leaves and its drinking safety are poorly understood. In this study, 85 tea varieties cultivated under the same conditions were investigated to analyze the seasonal variation of F content and it's relationships with F accumulation, aluminum (Al), calcium (Ca) and manganese (Mn) and hazard quotient (HQ) in young leaves (one bud and two leaves, YL) and mature leaves (canopy leaves, ML). The average F contents and accumulations were 350 mg kg−1 and 203 g ha−1 in YL, and they were 2451 mg kg−1 and 2578 g ha−1 in ML, respectively, with F mainly accumulated in ML. As the growing season progresses, the F content showed a gradual increase in YL, while a decrease in ML, inferring that F may be redistributed from mature leaves to young leaves. Additionally, the F content was quite different among tea varieties which are suitable for processing oolong tea, green tea, and black tea, with higher F accumulation in oolong tea varieties than in green and black tea varieties. Moreover, F content and accumulation could be obviously affected by the geographical origin of the tea tree varieties, with significantly higher F content in the varieties from F rich fluorite belts than other regions. Furthermore, F content and accumulation showed a significant positive correlation with the content of Al and Mn (p < 0.05). Based on a daily tea consumption of 8.7 g, the HQ was investigated to show that the proportion of tea leaves with HQ<1 made from spring, summer and autumn tender leaves of 85 varieties was 100 %, 90.6 % and 50.6 %, respectively, indicating that the tea with the best drinking safety comes from spring, followed by summer, and then autumn. This result suggests that it could be necessary to avoid planting tea trees in fluorite mining areas, choose low F tea tree varieties, and control the tenderness of fresh leaves in order to ensure the safety of tea drinking. • Fluorine (F) accumulation of 85 tea varieties in one growth cycle is studied. • The 85 tea varieties show a notable difference in genotype and F features. • F mainly accumulates in mature leaf and distributes to young leaf during growth. • F accumulation is related to tea suitability and leaf content of Al, Mn and Ca ions. • Young leaf tea has less hazard quotient and is safe for long-term drinking. [ABSTRACT FROM AUTHOR]

• The length of the designed PBS based on DNDHC-NCF is 1.38 cm, which solves the technical problems of long length of traditional PBS. • The operation bandwidth is 320 nm, covering S + C + L + U bands. • Is of great significance in promoting the application and development of DNDHC-NCF. In this paper, a polarization beam splitter（PBS） based on double nested dual-core negative-curvature fiber (DNHC-NCF) is designed. Two hollow cores are constructed by eight double nested cladding tubes arranged at certain angles. The influences of structural parameters on the performances of the DNHC-NCF PBS have been studied by using the finite element method (FEM). According to the simulation results, the structure of the proposed DNHC-NCF PBS is optimized. The final results indicate that the length of the proposed DNHC-NCF PBS is 1.38 cm and the extinction ratio (ER) is less than −20 dB in the wavelength range of 1.48–1.80 µm (320 nm, covers S + C + L + U bands). Besides, the higher order mode extinction ratio (HOMER) in the wavelength range of 1.3–1.71 µm is greater than 100, showing good single-mode characteristic as well. It is believed that the proposed double nested dual-core negative curvature fiber polarization beam splitter will have important applications in the fields of optical communication system. [ABSTRACT FROM AUTHOR]

Hemophilia A (HA) is a monogenic disease due to lack of the clotting factor VIII (FVIII). This deficiency may lead to spontaneous joint hemorrhages or life-threatening bleeding but there is no cure for HA until very recently. In this study, we derived induced pluripotent stem cells (iPSCs) from patients with severe HA and used transcription activator-like effector nickases (TALENickases) to target the factor VIII gene ( F8 ) at the multicopy ribosomal DNA (rDNA) locus in HA-iPSCs, aiming to rescue the shortage of FVIII protein. The results revealed that more than one copy of the exogenous F8 could be integrated into the rDNA locus. Importantly, we detected exogenous F8 mRNA and FVIII protein in targeted HA-iPSCs. After they were differentiated into endothelial cells (ECs), the exogenous FVIII protein was still detectable. Thus, it is showed that the multicopy rDNA locus could be utilized as an effective target site in patient-derived iPSCs for gene therapy. This strategy provides a novel iPSCs-based therapeutic option for HA and other monogenic diseases. [ABSTRACT FROM AUTHOR]