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D-glucaric acid is a promising platform compound used to synthesize many other value-added or commodity chemicals. The engineering of Escherichia coli for efficiently converting D -glucose to D-glucaric acid has been attempted for several years, with mixed sugar fermentation recently gaining growing interests due to the increased D-glucaric acid yield. Here, we co-expressed cscB , cscA , cscK , ino1 , miox , udh , and suhB in E. coli BL21 (DE3), functionally constructing an unreported route from sucrose to D-glucaric acid. Further deletion of chromosomal zwf , pgi , ptsG , uxaC , gudD , over-expression of glk , and use of a D -fructose-dependent translation control system for pgi enabled the strain to use sucrose as the sole carbon source while achieving a high product titer and yield. The titer of D-glucaric acid in M9 medium containing 10 g/L sucrose reached ~1.42 g/L, with a yield of ~0.142 g/g on sucrose. [ABSTRACT FROM AUTHOR]

Dipyridyl sulphide ligands 4-(pyridin-4-ylmethylthio)pyridine (abbreviated as L1) and 3-(pyridin-4-ylmethylthio)pyridine (abbreviated as L2) have been designed and used as μ- N , N -bridging linkages to construct coordination polymers with free –S–CH 2 – groups as secondary donor sites. By use solvent control method, coordination polymers {[Ag 3 SO 4 (L1) 3 ](Cl)·4.5H 2 O} ∞ ( 1 ), {[Ag 2 SO 4 (L1) 2 ]·6H 2 O·2CH 3 OH} ∞ ( 2 ), {[Ag 2 SO 4 (L2) 2 ]·H 2 O} ∞ ( 3 ) and {[Ag 4 (SO 4 ) 2 (L2) 4 ]·5H 2 O} ∞ ( 4 ) with different architectures were obtained. Complexes 1 , 3 and 4 feature 1D channel with different sizes and structures. Complex 1 exhibits guest exchange by THF and 1,4-dioxane, and Hg 2+ sorption ability from solution due to its relative larger channel and available bonding sites of –S– exposed to the channel region. All complexes have been characterized through single-crystal and powder X-ray diffraction (PXRD), FT-IR spectra, X-ray photoelectron spectroscopy (XPS), elemental and thermogravimetric analyses. The guest exchange and Hg 2+ sorption were monitored and identified, and the structure-property relationship of coordination polymers 1–4 are discussed. [ABSTRACT FROM AUTHOR]