1. Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues
- Author
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Yoo, Keon Hee, Jang, In Keun, Lee, Myoung Woo, Kim, Hyo Eun, Yang, Mal Sook, Eom, Youngwoo, Lee, Jong Eun, Kim, Young Jin, Yang, Seong Kyu, Jung, Hye Lim, Sung, Ki Woong, Kim, Cheol Woo, and Koo, Hong Hoe
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STEM cells , *IMMUNOLOGICAL adjuvants , *COMPARATIVE studies , *ANTI-inflammatory agents , *BONE marrow , *CYTOKINES , *IMMUNOSUPPRESSIVE agents , *PHYTOHEMAGGLUTININS - Abstract
Abstract: Mesenchymal stem cells (MSCs), which evoke only minimal immune reactivity, may have anti-inflammatory and immunomodulatory effects. In this study, we conducted a comparative analysis of the immunomodulatory properties of MSCs derived from adult human tissues including bone marrow (BM), adipose tissues (AT), umbilical cord blood (CB), and cord Wharton’s jelly (WJ). Using a multiple cytokine detection assay, we showed that there were no significant differences in levels of secreted factors from non-stimulated MSCs. We compared the immunosuppressive effect of BM-MSCs, AT-MSCs, CB-MSCs, and WJ-MSCs on phytohemagglutinin-induced T-cell proliferation. AT-MSCs, CB-MSCs, and WJ-MSCs effectively suppressed mitogen-induced T-cell proliferation as effectively as did BM-MSCs. Levels of interferon (IFN)-γ and tumor necrosis factor (TNF)-α secreted from activated T-cells increased over time, but these levels were significantly reduced when cocultured with each type of MSCs. In addition, the expression of hepatocyte growth factor, IL-10, transforming growth factor-β1, cyclooxygenase (COX)-1, and COX-2 were unchanged in MSCs treated with IFN-γ and/or TNF-α, while indoleamine 2,3-dioxygenase (IDO) expression increased. IFN-γ and/or TNF-α produced by activated T-cells were correlated with induction of IDO expression by MSCs, which, in turn, suppressed T-cell proliferation. These findings suggest that MSCs derived from AT, CB, or WJ could be substituted for BM-MSCs for treatment of allogeneic conflicts. [Copyright &y& Elsevier]
- Published
- 2009
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