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The gastropod mollusk Limax flavus , one of the most widespread pests in China, is used to treat infectious diseases in traditional Chinese medicine. However, little genomic information is available for this non-model species. In this study, the whole-body transcriptome of L. flavus was sequenced using next generation sequencing technology. A total of 6.81 Gb clean reads were obtained, which were assembled into 150,766 transcripts with 132,206 annotated unigenes. Functionally classification assigned 30,542 unigenes to 56 Gene Ontology terms, 16,745 unigenes were divided into 26 euKaryotic Ortholog Groups of proteins categories, and 13,854 unigenes were assigned to 230 Kyoto Encyclopedia of Genes and Genomes pathways. Furthermore, we identified 17,251 simple sequence repeats and several kinds of antimicrobial peptide and protein (AMPs) genes. The transcriptome data of L. flavus will provide a valuable genomic resource for further studies on this species, and the AMPs identified in L. flavus will support its medical potential. • The gastropod mollusk Limax flavus is used to treat infectious diseases in traditional Chinese medicine. • Little genomic information is available for the non-model species L. flavus. • This study is the first whole transcriptome analysis of L. flavus. • It will help explore the genome of L. flavus and promote the medicinal use of L. flavus. [ABSTRACT FROM AUTHOR]

The emergence of antibiotic resistance has severely impaired the treatment of infections caused by Pseudomonas aeruginosa. There are few studies related to comparing the antibiotics resistance mechanisms of P. aeruginosa against different antibiotics. In this study, RNA sequencing was used to investigate the differences of transcriptome between wild strain and four antibiotics resistant strains of P. aeruginosa PAO1 (polymyxin B, ciprofloxacin, doxycycline, and ceftriaxone). Compared to the wild strain, 1907, 495, 2402, and 116 differentially expressed genes (DEGs) were identified in polymyxin B, ciprofloxacin, doxycycline, and ceftriaxone resistant PAO1, respectively. After analysis of genes related to antimicrobial resistance, we found genes implicated in biofilm formation (pelB , pelC , pelD , pelE , pelF , pelG , algA , algF , and alg44) were significantly upregulated in polymyxin B-resistant PAO1, efflux pump genes (mexA , mexB , oprM) and biofilm formation genes (pslJ , pslK and pslN) were upregulated in ciprofloxacin-resistant PAO1; other efflux pump genes (mexC , mexD , oprJ) were upregulated in doxycycline-resistant PAO1; ampC were upregulated in ceftriaxone-resistant PAO1. As a consequence of antibiotic resistance, genes related to virulence factors such as type Ⅱ secretion system (l asA , lasB and piv) were significantly upregulated in polymyxin B-resistant PAO1, and type Ⅲ secretion system (exoS , exoT , exoY , exsA , exsB , exsC , exsD , pcrV , popB , popD , pscC , pscE , pscG , and pscJ) were upregulated in doxycycline-resistant PAO1. While, ampC were upregulated in ceftriaxone-resistant PAO1. In addition, variants were obtained in wild type and four antibiotics resistant PAO1. Our findings provide a comparative transcriptome analysis of antibiotic resistant mutants selected by different antibiotics, and might assist in identifying potential therapeutic strategies for P. aeruginosa infection. • Upregulated pelB-G , algA , algF and alg44 might result in polymyxin B resistance to PAO1. • Enhanced expression of mexA , mexB and oprM might contribute to ciprofloxacin resistance to PAO1. • Upregulated mexC , mexD , oprJ and T3SS genes might lead to resistance and increased virulence in doxycycline resistant PAO1. • The ceftriaxone resistance to PAO1 might be due to upregulated ampC. [ABSTRACT FROM AUTHOR]