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SHP1 is a non-receptor protein tyrosine phosphatase that is widely expressed in hematopoietic cells such as white blood cells, neutrophils, and immune cells. SHP1 can regulate the occurrence and differentiation of immune cells and plays an important role as a tumor suppressor. Previous studies have suggested that SHP2, the homologous protein of phosphatase SHP1, can undergo liquid–liquid phase separation (LLPS). Therefore, in this study, we investigated if SHP1 is also capable of LLPS. To the best of our knowledge, our study is the first to reveal that SHP1 has the ability to undergo LLPS. In addition, we identified an important residue, SHP1-R360E, that can completely inhibit the LLPS ability of SHP1, but this mutation has no remarkable effect on SHP1's enzymatic activity. This allows us to explore the phosphatase activity and phase separation ability of SHP1 separately, providing a basis for future exploration of the phase separation mechanism of phosphatases. • SHP1 can undergo liquid–liquid phase separation(LLPS). • The PTP domain is responsible for SHP1 LLPS. • The SHP1 mutant, K358E/R360E, can perturb LLPS and catalytic ability. • SHP1-R360E can abolish the LLPS ability of SHP1 without affecting SHP1's enzymatic activity. [ABSTRACT FROM AUTHOR]