Search

Experiments on random-bred white male rats have demonstrated the activation of induced lipid peroxidation in red cell membranes, elevation on the basal level of plasma lipid peroxides, a decrease in the content of alpha-tocopherol in plasma and red blood cell membranes, considerable shifts in the content of esterified and free cholesterol in plasma and red blood cell membranes under prolonged acoustic stress (91 dB). Administration of alpha-tocopheryl acetate in a dose of 1 mg/kg exerted a beneficial effect on the test parameters under prolonged acoustic stress.

The membrane conductivity for K+ and Ca+2 ions was studied on bilayer phospholipid membranes formed by phospholipids extracted from the rat liver 1 hour, 1, 3, 7, and 15 days after burning. A pronounced increase in the membrane conductivity was noted. The most potent effects were seen by the 1t, 3d and 7th day of the experiment and the conductivity value varied depending on the medium pH. The process was accompanied by lipid peroxidation increase. Intraperitoneal administration of alpha-tocopherol at 1 mg/kg dose immediately after burning, followed by injections on the 3d, 7th and 12th day, normalized the characteristics studied. Bilayer membranes formed by healthy rat liver phospholipids previously added with an appropriate amount of methyl oleate or cumylhydroperoxide demonstrated higher conductivity as compared to the normal.

The contents of Cu, Zn-superoxide dismutase and catalase isolated and purified from the rat liver at the terminal stages of alloxan diabetes were decreased by 40% and 15%, respectively, as compared to the control. It can be concluded that the decrease in superoxide dismutase and catalase activity in experimental alloxan diabetes is mainly connected with the decline in the content of these proteins at the terminal stages of the disease, this, probably, being the result of DNA degradation and RNA transport disturbances under the effect of oxygen active forms.