1. Monitoring lactic acid concentrations by infrared spectroscopy: A new developed method for Lactobacillus fermenting media with potential food applications
- Author
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Carmen Socaciu, Simona Man, Floricuta Ranga, Sevastița Muste, Vlad Mureșan, Dan Cristian Vodnar, Adriana Paucean, and Florinela Fetea
- Subjects
Lactobacillus casei ,biology ,Animal food ,Microorganism ,010401 analytical chemistry ,food and beverages ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,01 natural sciences ,0104 chemical sciences ,Lactic acid ,chemistry.chemical_compound ,0404 agricultural biotechnology ,chemistry ,Lactobacillus ,Fermentation ,Food science ,Bacteria ,Lactobacillus plantarum ,Food Science - Abstract
The lactic acid bacteria are key microorganisms for the production and preservation of fermented dairy products, cheeses, sourdough bread, and lacto-fermented vegetables. This study was developed to monitor lactic acid produced by Lactobacillus plantarum ATCC 8014 and Lactobacillus casei ATCC 393, as single strains and combined, in fermenting media by Fourier Transform Infrared Spectroscopy coupled to multivariate statistical analysis. Media containing different mixtures of carbohydrates were chosen as model fermenting media for monitoring lactic acid concentration by infrared spectroscopy, due to the fact that vegetable and animal food matrices could contain different carbohydrates as carbon sources. Three different types of media were obtained by adding different carbohydrates to a basic MRS medium. HPLC was used as reference method for lactic acid quantification. The calibration set (n=36) was used for building model, while a validation set (n=13) for testing the robustness of the developed model. The ...
- Published
- 2017
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