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Start Over Descriptor "Nitrobenzoates" Publisher akademie verlag

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1 results on '"Nitrobenzoates"'

1. [Specific cleavage of the cysteine-peptide bond of thermitase].

Author

Hausdorf G and Krüger K

Subjects
Amino Acids analysis, Chromatography, Gel, Dansyl Compounds analysis, Electrophoresis, Polyacrylamide Gel methods, Hydrolysis, Molecular Weight, Nitrobenzoates, Peptide Fragments analysis, Sulfhydryl Compounds analysis, Cysteine analysis, Endopeptidases analysis, Serine Endopeptidases
Abstract

Cyanylation and N-cysteine peptide bond cleavage of thermitase using 2-nitro-5-thiocyanobenzoic acid results in the formation of two peptide fragments (mol. wt. 20 000 and 10 000) in high yield (about 90%). These peptides are well separated by gel filtration on Sephadex G-75 in the preparative scale.

Published
1984

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