1. Purification of serum albumin with dye-ligand adsorption chromatography.
- Author
-
Su ZG, Jiang W, Feng PS, and Gao KY
- Subjects
- Adsorption, Humans, Hydrogen-Ion Concentration, Ligands, Molecular Structure, Temperature, Chromatography, Agarose methods, Coloring Agents, Serum Albumin isolation & purification
- Abstract
Dye molecules were coupled to agarose gel beads to form blue, red and yellow chromatography media for the separation of serum albumin. The adsorption properties of five dye-ligands were compared and the effects of temperature, pH, protein concentration and salt concentration were investigated. Human serum albumin (HSA) was purified from human placental plasma by a single step of dye-ligand chromatography under suitable operating conditions. The product was electrophoretically pure, and the elution recovery was as high as 95%. This new technique is superior to the conventional salting-out process.
- Published
- 1991