1. Multiplexed Cell Signaling Analysis of Human Breast Cancer Applications for Personalized Therapy
- Author
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Donato Nitti, Lance A. Liotta, Petricoin Ef rd, Belluco C, J. Deng, Laura J. Esserman, Julie Wulfkuhle, Mammano E, Sandra M. Swain, R. Speer, Espina, Pierobon M, Laird J, and S. X. Yang
- Subjects
Proteomics ,Cell signaling ,Breast Neoplasms ,Computational biology ,Biology ,Bioinformatics ,Biochemistry ,Erlotinib Hydrochloride ,Breast cancer ,medicine ,Cluster Analysis ,Humans ,Phosphorylation ,Protein Kinase Inhibitors ,Laser capture microdissection ,Lasers ,Liver Neoplasms ,Intracellular Signaling Peptides and Proteins ,Phosphoproteomics ,Receptor Protein-Tyrosine Kinases ,General Chemistry ,Phosphoproteins ,medicine.disease ,Quinazolines ,Protein microarray ,Female ,Signal transduction ,Microdissection ,Protein Kinases ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
Phosphoprotein driven cellular signaling events represent most of the new molecular targets for cancer treatment. Application of reverse-phase protein microarray technology for the study of ongoing signaling activity within breast tumor specimens holds great potential for elucidating and profiling signaling activity in real-time for patient-tailored therapy. Analysis of laser capture microdissection primary human breast tumors and metastatic lesions reveals pathway specific profiles and a new way to classify cancer based on functional signaling portraits. Moreover, the data demonstrate the requirement of laser capture microdissection for analysis and reveal the metastasis-specific changes that occur within a new microenvironment. Analysis of biopsy material from clinical trials for targeted therapeutics demonstrates the feasibility and utility of comprehensive signal pathway activation profiling for molecular analysis.
- Published
- 2008