1. Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of alpha-Galactosyl Residues
- Author
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Rodrigo Pedro Soares, Rafael Ramiro de Assis, Ana Claudia Torrecilhas, Sara Lopes dos Santos, Silvane M. F. Murta, Alexandre F. Marques, Gustavo F. Freitas, Marcele Neves Rocha, Felipe A. Moura e Castro, Igor C. Almeida, Alvaro J. Romanha, Fiocruz MS, Universidade Federal de São Paulo (UNIFESP), and Univ Texas El Paso
- Subjects
Chagas disease ,Glycosylphosphatidylinositols ,Trypanosoma cruzi ,Nitric oxide ,chemistry.chemical_compound ,Species Specificity ,Virology ,parasitic diseases ,medicine ,Animals ,Differential expression ,Nitrites ,chemistry.chemical_classification ,Strain (chemistry) ,biology ,Mucin ,Mucins ,Articles ,Carbohydrate ,medicine.disease ,biology.organism_classification ,Infectious Diseases ,Enzyme ,chemistry ,Biochemistry ,Macrophages, Peritoneal ,Cytokines ,Parasitology - Abstract
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) National Institutes of Health Biomolecule Analysis Core Facility at the Border Biomedical Research Center/Biology/UTEP University of Texas at El Paso (National Institutes of Health) The glycosylphosphatidylinositol (GPI)-anchored mucins of Trypanosoma cruzi trypomastigotes play an important immunomodulatory role during the course of Chagas disease. Here, some biological activities of tGPI-mucins from four T cruzi isolates, including benznidazole-susceptible (BZS-Y), benznidazole-resistant (BZR-Y), CL, and Colombiana, were evaluated. GPI-mucins were able to differentially trigger the production of interleukin-12 and nitric oxide in BALB/c macrophages and modulate LLC-MK2 cell invasion. the significance of these variations was assessed after analysis of the terminal alpha-galactosyl residues. Enzymatic treatment with alpha-galactosidase indicated a differential expression of O-linked alpha-galactosyl residues among the strains, with higher expression of this sugar in BZS-Y and BZR-Y T cruzi populations followed by Colombiana and CL. Unweighted pair group method analysis of the carbohydrate anchor profile and biological parameters allowed the clustering of two groups. One group includes Y and CL strains (T cruzi II and VI), and the other group is represented by Colombiana strain (T. cruzi I). Fiocruz MS, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG, Brazil Universidade Federal de São Paulo, UNIFESP, Dept Ciencias Biol, Diadema, SP, Brazil Univ Texas El Paso, Dept Biol Sci, Border Biomed Res Ctr, El Paso, TX 79968 USA Universidade Federal de São Paulo, UNIFESP, Dept Ciencias Biol, Diadema, SP, Brazil CNPq: 305042/2010-6 CNPq: 552072/2009-5 CNPq: PAPES-IV-400138/2006-9 CNPq: PDJ-150880/2005-7 National Institutes of Health: 1R01AI070655-04 National Institutes of Health: 3R01AI070655-04S1 National Institutes of Health: 2G12RR008124-16A1 National Institutes of Health: 2G12RR008124-16A1S1 Biomolecule Analysis Core Facility at the Border Biomedical Research Center/Biology/UTEP University of Texas at El Paso (National Institutes of Health): 2G12RR008124-16A1 Biomolecule Analysis Core Facility at the Border Biomedical Research Center/Biology/UTEP University of Texas at El Paso (National Institutes of Health): 2G12RR008124-16A1S1 Biomolecule Analysis Core Facility at the Border Biomedical Research Center/Biology/UTEP University of Texas at El Paso (National Institutes of Health): G12MD007592 Web of Science
- Published
- 2012