1. Detection of Clostridioides difficile by Real-time PCR in Young Children Does Not Predict Disease
- Author
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James D. Chappell, Ashley K. Sherman, Natasha B. Halasa, Christopher J. Harrison, Ferdaus Hassan, Geoffrey A. Weinberg, L. Clifford McDonald, Peter G. Szilagyi, Mary E Wikswo, Daniel C. Payne, Christopher R. Polage, Janet A. Englund, Rangaraj Selvarangan, Eileen J. Klein, and Barbara A. Pahud
- Subjects
medicine.medical_specialty ,business.industry ,General Medicine ,Disease ,Clostridium difficile ,C difficile ,Pediatrics ,Asymptomatic ,law.invention ,03 medical and health sciences ,Diarrhea ,0302 clinical medicine ,Real-time polymerase chain reaction ,law ,030225 pediatrics ,Internal medicine ,Pediatrics, Perinatology and Child Health ,medicine ,030212 general & internal medicine ,medicine.symptom ,business ,Clostridioides ,Polymerase chain reaction - Abstract
OBJECTIVES: Diagnosing Clostridioides difficile infections in young children with high asymptomatic colonization is challenging. We compared the frequency of C difficile detection by polymerase chain reaction (PCR) in healthy control (HC) children with those with acute gastroenteritis (AGE) and evaluated fecal-lactoferrin and organism load as possible indicators of true C difficile infection disease. METHODS: Stool was collected from children RESULTS: Of 524 stools collected from 524 children (250 with AGE, 274 HCs), C difficile was detected less in children with AGE (14%, 36 of 250) than in HCs (28%, 76 of 274) stools (P < .0001). Among infants CONCLUSIONS: HC children
- Published
- 2020
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