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Your search keyword '"Mashino K"' showing total 2 results
Start Over Author "Mashino K" Publisher american association for cancer research
2 results on '"Mashino K"'

1. Expression of chemokine receptor CCR7 is associated with lymph node metastasis of gastric carcinoma.

Author

Mashino K, Sadanaga N, Yamaguchi H, Tanaka F, Ohta M, Shibuta K, Inoue H, and Mori M

Subjects
Actins metabolism, Aged, Calcium metabolism, Chemokine CCL21, Chemokines, CC pharmacology, Chemotaxis drug effects, Female, Humans, Lymphatic Metastasis, Male, Neoplasm Invasiveness, RNA, Messenger biosynthesis, RNA, Messenger genetics, Receptors, CCR7, Receptors, Chemokine genetics, Tumor Cells, Cultured, Lymph Nodes pathology, Receptors, Chemokine biosynthesis, Stomach Neoplasms metabolism, Stomach Neoplasms pathology
Abstract

The interactions of chemokine receptor CCR7 and its ligands are essential for migration of lymphocytes and dendritic cells to lymph nodes. In this study, we found that 4 of 6 (67%) gastric carcinoma cell lines tested expressed functional CCR7 for the chemokine CCL21/6Ckine, as demonstrated by calcium mobilization and actin polymerization assays. Moreover, we also showed that signaling through CCR7 induced chemotactic and invasive responses in CCR7-positive gastric carcinoma cells. In clinical samples, immunohistochemical assay showed that CCR7-positive carcinoma cells were detected in 42 of 64 (66%) cases and a significant difference in both lymph node metastasis (P < 0.001) and lymphatic invasion (P < 0.001) between CCR7-positive and -negative cases. Patients with CCR7-positive tumors had a significantly poorer prognosis than those with CCR7-negative tumors (P < 0.05). Stepwise regression analysis revealed that the most important factor related to lymph node metastasis was the expression of CCR7. These results indicated that CCR7 and its ligands interaction is associated with preferential lymph node metastasis of gastric carcinoma.

Published
2002

2. Dendritic cell vaccination with MAGE peptide is a novel therapeutic approach for gastrointestinal carcinomas.

Author

Sadanaga N, Nagashima H, Mashino K, Tahara K, Yamaguchi H, Ohta M, Fujie T, Tanaka F, Inoue H, Takesako K, Akiyoshi T, and Mori M

Subjects
Aged, Aged, 80 and over, Antigens, Neoplasm analysis, CA-19-9 Antigen analysis, Cancer Vaccines immunology, Carcinoembryonic Antigen analysis, Cytotoxicity, Immunologic, Female, Gastrointestinal Neoplasms metabolism, Gastrointestinal Neoplasms prevention & control, Humans, Immunohistochemistry, Male, Middle Aged, Treatment Outcome, Dendritic Cells immunology, Gastrointestinal Neoplasms immunology, Immunotherapy, Adoptive, Neoplasm Proteins immunology, Serpins
Abstract

The MAGE gene is selectively expressed in cancer tissues such as melanoma or gastrointestinal carcinomas, whereas no expression is observed in normal tissues except testis. There are several reports of successful induction of HLA class I-restricted antitumor CTLs using MAGE peptides, and some clinical trials with these immunogenic peptides were reported as effective for some patients with malignant melanoma. However, there are no similar studies in gastrointestinal carcinomas, which are important neoplasms. Autologous dendritic cells (DCs) were generated ex vivo and were pulsed with MAGE-3 peptide, depending on the patient's HLA haplotype (HLA-A2 or A24). Patients were immunized with DC pulsed with MAGE-3 peptide every 3 weeks at four times. Twelve patients with advanced gastrointestinal carcinoma (six stomach, three esophagus, and three colon) were treated, and no toxic side effects were observed. Peptide-specific CTL responses after vaccination were observed in four of eight patients. Improvement in performance status was recognized in four patients. Tumor markers decreased in seven patients. In addition, minor tumor regressions evidenced by imaging studies were seen in three patients. These results suggested that DC vaccination with MAGE-3 peptide is a safe and promising approach in the treatment of gastrointestinal carcinomas.

Published
2001

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